https://scholars.lib.ntu.edu.tw/handle/123456789/638024
標題: | ShyA, a membrane protein for proper septation of hyphae in Streptomyces | 作者: | Tseng, Shun-Fu Huang, Tzu-Wen Chen, Carton W Chern, Ming-Kai Tam, Ming F SHU-CHUN TENG |
關鍵字: | Actinomycetes | Hyphae | Membrane protein | Spore | Streptomyces | 公開日期: | 5-五月-2006 | 卷: | 343 | 期: | 2 | 來源出版物: | Biochemical and biophysical research communications | 摘要: | The life cycle of Streptomyces involves the formation of filamentous substrate and aerial hyphae. Following cessation of growth of an aerial hypha, multiple septation occurs at the tip to produce a chain of unigenomic spores. A gene, shyA, which influences several aspects of this growth, was isolated and partially characterized in Streptomyces coelicolor. The gene product is a representative of a well-conserved family of small actinomycete proteins. The shyA mutant sporulates normally but displays hyper septum formation and altered spore-chain morphology. Biochemical separation experiments and immunofluorescence staining demonstrated that the shyA gene product locates at cell membranes. Moreover, yeast two-hybrid screen and GST-pull-down assay showed that ShyA can interact with itself. Altogether, ShyA belongs to a new family of membrane-associated proteins which plays a role in morphological differentiation in actinomycetes. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638024 | ISSN: | 0006-291X | DOI: | 10.1016/j.bbrc.2006.02.178 |
顯示於: | 微生物學科所 |
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