https://scholars.lib.ntu.edu.tw/handle/123456789/638291
Title: | The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit | Authors: | Durfee, T Becherer, K Chen, P L Shiou-Hwei Yeh Yang, Y Kilburn, A E Lee, W H Elledge, S J |
Keywords: | Cell-cycle regulation | Protein phosphatase 1 | Protein-protein interaction | Retinoblastoma | Two-hybrid system | Issue Date: | Apr-1993 | Journal Volume: | 7 | Journal Issue: | 4 | Source: | Genes & development | Abstract: | The retinoblastoma protein (p110RB) interacts with many cellular proteins in complexes potentially important for its growth-suppressing function. We have developed and used an improved version of the yeast two-hybrid system to isolate human cDNAs encoding proteins able to bind p110RB. One clone encodes a novel type 1 protein phosphatase catalytic subunit (PP-1 alpha 2), which differs from the originally defined PP-1 alpha by an amino-terminal 11-amino-acid insert. In vitro-binding assays demonstrated that PP-1 alpha isoforms preferentially bind the hypophosphorylated form of p110RB. Moreover, similar p110RB sequences are required for binding PP-1 alpha 2 and SV40 large T antigen. Cell cycle synchrony experiments revealed that this association occurs from mitosis to early G1. The implications of these findings on the regulation of both proteins are discussed. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638291 | ISSN: | 0890-9369 | DOI: | 10.1101/gad.7.4.555 |
Appears in Collections: | 微生物學科所 |
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