https://scholars.lib.ntu.edu.tw/handle/123456789/638298
標題: | Optical sectioning fluorescence microscopy using variable metalens | 作者: | Chu, Cheng Hung Vyas, Sunil Kuo, Hsin Yu Chia, Yu Hsin Chen, Mu Ku YI-YOU HUANG Tsai, Din Ping YUAN LUO |
關鍵字: | fluorescence microscopy | metasurface | optical sectioning | 公開日期: | 1-一月-2023 | 卷: | 12608 | 來源出版物: | Proceedings of SPIE - The International Society for Optical Engineering | 摘要: | Fluorescence microscopy is a common method to observe the structural features of biological samples. Conventional tunable lenses are usually used to axially scan the volumetric samples for obtaining fluorescence optical sectioning images. However, they suffer from spherical aberration and distortions. In this work, we propose a compact imaging system with optical sectioning capability based on Moiré metalens which consists of two complementary phase metasurfaces to perform fluorescence bio-imaging applications in visible regions. The focal length of system can be tuned from 10 mm to 125 mm at the wavelength of 532 nm by changing mutual angles between two metasurfaces. In addition, the speckle illumination HiLo microscope is used to reduce the effect of out-of-focus light scattering. To demonstrate the optical sectioning capability of our system, the labeled beads as well as ex vivo mice intestine tissue samples are imaged. The presented design of varifocal metalens is anticipated to realize important applications in fluorescence microscopy and endoscopy. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638298 | ISBN: | 9781510663435 | ISSN: | 0277786X | DOI: | 10.1117/12.3007925 |
顯示於: | 醫療器材與醫學影像研究所 |
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