https://scholars.lib.ntu.edu.tw/handle/123456789/638309
Title: | Ribosomal protein RPS-14 modulates let-7 microRNA function in Caenorhabditis elegans | Authors: | SHIH-PENG CHAN Slack, Frank J |
Keywords: | C. elegans | let-7 | MicroRNA | Ribosome | RPS-14 | Stem cell | Issue Date: | 1-Oct-2009 | Journal Volume: | 334 | Journal Issue: | 1 | Source: | Developmental biology | Abstract: | The let-7 microRNA (miRNA) regulates developmental timing at the larval-to-adult transition in Caenorhabditis elegans. Dysregulation of let-7 results in irregular hypodermal and vulval development. Disrupted let-7 function is also a feature of human lung cancer. However, little is known about the mechanism and co-factors of let-7. Here we demonstrate that ribosomal protein RPS-14 is able to modulate let-7 function in C. elegans. The RPS-14 protein co-immunoprecipitated with the nematode Argonaute homolog, ALG-1. Reduction of rps-14 gene expression by RNAi suppressed the aberrant vulva and hypodermis development phenotypes of let-7(n2853) mutant animals and the mis-regulation of a reporter bearing the lin-41 3'UTR, a well established let-7 target. Our results indicate an interactive relationship between let-7 miRNA function and ribosomal protein RPS-14 in regulation of terminal differentiation that may help in understanding the mechanism of translational control by miRNAs. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638309 | ISSN: | 00121606 | DOI: | 10.1016/j.ydbio.2009.07.011 |
Appears in Collections: | 微生物學科所 |
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