https://scholars.lib.ntu.edu.tw/handle/123456789/638371
標題: | Lipid raft interacting galectin 8 regulates primary ciliogenesis | 作者: | Syu, Jhan-Jhang Chang, Chieh-Hsiang Chang, Pei-Yu Liu, Chia-Hsiung Yu, Chia-Jung TZUU-SHUH JOU |
關鍵字: | caveolin 1; galectin 8; ganglioside; lipid raft; primary cilia; transition zone | 公開日期: | 十二月-2023 | 卷: | 37 | 期: | 12 | 來源出版物: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 摘要: | Primary cilium is a specialized sensory organelle that transmits environmental information into cells. Its length is tightly controlled by various mechanisms such as the frequency or the cargo size of the intraflagellar transport trains which deliver the building materials such as tubulin subunits essential for the growing cilia. Here, we show the sialoglycan interacting galectin 8 regulates the process of primary ciliogenesis. As the epithelia become polarized, there are more galectin 8 being apically secreted and these extracellular galectin 8 molecules apparently bind to a lipid raft enriched domain at the base of the primary cilia through interacting with lipid raft components, such as GD3 ganglioside and scaffold protein caveolin 1. Furthermore, the binding of galectin 8 at this critical region triggers rapid growth of primary cilia by perturbing the barrier function of the transition zone (TZ). Our study also demonstrates the functionality of this barrier depends on intact organization of lipid rafts at the cilia as genetically knockout of Cav1 and pharmacologically inhibition of lipid raft both phenocopy the effect of apical addition of recombinant galectin 8; that is, rapid elongation of primary cilia and redistribution of cilia proteins from TZ to the growing axoneme. Indeed, as cilia elongated, endogenous galectin 8, caveolin 1, and TZ component, TMEM231, also transited from the TZ to the growing axoneme. We also noted that the interaction between caveolin 1 and TMEM231 could be perturbed by exogenous galectin 8. Taken together, we proposed that galectin 8 promoted primary cilia elongation through impeding the barrier function of the TZ by interfering with the interaction between caveolin 1 and TMEM231. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638371 | ISSN: | 08926638 | DOI: | 10.1096/fj.202301943R |
顯示於: | 臨床醫學研究所 |
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