https://scholars.lib.ntu.edu.tw/handle/123456789/638499
標題: | Development of novel microsatellite markers to analyze the genetic structure of dog populations in Taiwan | 作者: | Lai, Fang Yu Lin, Yu-Chen SHIH-TORNG DING Chang, Chi Sheng Chao, Wi Lin PEI-HWA WANG |
關鍵字: | Dog | Novel Microsatellite Marker | Population Genetic Analysis | 公開日期: | 1-九月-2022 | 卷: | 35 | 期: | 9 | 來源出版物: | Animal Bioscience | 摘要: | Objective: Alongside the rise of animal-protection awareness in Taiwan, the public has been paying more attention to dog genetic deficiencies due to inbreeding in the pet market. The goal of this study was to isolate novel microsatellite markers for monitoring the genetic structure of domestic dog populations in Taiwan. Methods: A total of 113 DNA samples from three dog breeds—beagles (BEs), bichons (BIs), and schnauzers (SCs)—were used in subsequent polymorphic tests applying the 14 novel microsatellite markers that were isolated in this study. Results: The results showed that the high level of genetic diversity observed in these novel microsatellite markers provided strong discriminatory power. The estimated probability of identity (P(ID)) and the probability of identity among sibs (P(ID)sib) for the 14 novel microsatellite markers were 1.7×10–12 and 1.6×10–5, respectively. Furthermore, the power of exclusion for the 14 novel microsatellite markers was 99.98%. The neighbor-joining trees constructed among the three breeds indicated that the 14 sets of novel microsatellite markers were sufficient to correctly cluster the BEs, BIs, and SCs. The principal coordinate analysis plot showed that the dogs could be accurately separated by these 14 loci based on different breeds; moreover, the Beagles from different sources were also distinguished. The first, the second, and the third principal coordinates could be used to explain 44.15%, 26.35%, and 19.97% of the genetic variation. Conclusion: The results of this study could enable powerful monitoring of the genetic structure of domestic dog populations in Taiwan. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638499 | ISSN: | 27650189 | DOI: | 10.5713/ab.21.0519 |
顯示於: | 動物科學技術學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。