https://scholars.lib.ntu.edu.tw/handle/123456789/638504
標題: | Age and dietary restriction modulate mitochondrial quality in quadriceps femoris muscle of male mice | 作者: | Zhang, Ting Rui Chiang, Chun Hsien Hsu, Tzu Chieh Wang, Chih Yun CHING-YI CHEN |
關鍵字: | Ageing | Dietary restriction | Mitochondrial homeostasis | Quadriceps femoris muscle | 公開日期: | 1-一月-2024 | 來源出版物: | Biogerontology | 摘要: | Dietary restriction (DR) is a potential intervention for ameliorating ageing-related damages. Mitochondrial quality control is the key mechanism for regulating cellular functions in skeletal muscle. This study aimed to explore the effect of age and DR on the homeostasis of mitochondrial quality control in skeletal muscle. To study the effect of age on mitochondrial homeostasis, young (3 months old) male C57BL/6J mice were fed ad libitum (AL) until 7 (Young), 14 (Middle), and 19 months (Aged) of age. For the DR intervention, 60% of AL intake was given to the mice at 3 months of age until they reached 19 months of age (16 months). The quadriceps femoris muscle was collected for further analysis. Significant changes in the skeletal muscle were noticed during the transition between middle age and the elderly stages. An accumulation of collagen was observed in the muscle after middle age. Compared with the Middle muscle, Aged muscle displayed a greater expression of VDAC, and lower expressions of mitochondrial dynamic proteins and OXPHOS proteins. The DR intervention attenuated collagen content and elongated the sarcomere length in the skeletal muscle during ageing. In addition, DR adjusted the abnormalities in mitochondrial morphology in the Aged muscle. DR downregulated VDAC expression, but upregulated OPA1 and DRP1 expressions. Taken together, greater pathological changes were noticed in the skeletal muscle during ageing, especially in the transition between middle age and the elderly, whereas early-onset DR attenuated the muscular ageing via normalising partial functions of mitochondria. |
URI: | https://www.scopus.com/record/display.uri?eid=2-s2.0-85181528364&origin=resultslist https://scholars.lib.ntu.edu.tw/handle/123456789/638504 |
ISSN: | 13895729 | DOI: | 10.1007/s10522-023-10086-3 |
顯示於: | 動物科學技術學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。