https://scholars.lib.ntu.edu.tw/handle/123456789/638575
標題: | Spherical aberration correction in multiphoton fluorescence imaging using objective correction collar | 作者: | Lo, Wen Sun, Yen SUNG-JAN LIN SHIOU-HWA JEE Dong, Chen-Yuan |
關鍵字: | Fluorescence | Multiphoton | Objective correction collar | Rat tail tendon | Second harmonic generation | Skin | Spherical aberration | 公開日期: | 2005 | 卷: | 10 | 期: | 3 | 來源出版物: | Journal of biomedical optics | 摘要: | Multiphoton microscopy has evolved into a powerful bioimaging tool in three dimensions. However, the ability to image biological specimens in-depth can be hindered by sample spherical aberration and scattering. These two phenomena can result in the degradation of image resolution and the loss of detected multiphoton signal. In this work, we use the correction collar (for cover glass thickness) associated with a water immersion objective in an attempt to improve multiphoton imaging. In the two samples we examined (human skin and rat tail tendon), we found that while the improvement in image resolution was not visible qualitatively, the measured axial fluorescence or second harmonic generation signal profiles indicate that the use of the correction collar can help to improve the detected multiphoton signals. The maximum increases are 36% and 57% for the skin (sulforhodamine B fluorescence) and tendon (second harmonic generation) specimens, respectively. Our result shows that for in-depth multiphoton imaging, the correction collar may be used to correct for spherical aberration. However, each tissue type needs to be examined to determine the optimal correction collar setting to be used. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638575 | ISSN: | 1083-3668 | DOI: | 10.1117/1.1924614 |
顯示於: | 醫學工程學研究所 |
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