https://scholars.lib.ntu.edu.tw/handle/123456789/638813
標題: | Production of bacterial cellulose (BC)/nisin composite with enhanced antibacterial and mechanical properties through co-cultivation of Komagataeibacter xylinum and Lactococcus lactis subsp. lactis | 作者: | Huang, Yi-Cheng Khumsupan, Darin Lin, Shin-Ping Santoso, Shella Permatasari Hsu, Hsien-Yi KUAN-CHEN CHENG |
關鍵字: | Bacterial cellulose; Co-cultivation; Komagataeibacter xylinum; Lactococcus lactis subsp. lactis; Nisin | 公開日期: | 26-十二月-2023 | 卷: | 258 | 期: | Pt 2 | 來源出版物: | International journal of biological macromolecules | 摘要: | By employing co-cultivation technique on Komagataeibacter xylinum and Lactococcus lactis subsp. lactis, bacterial cellulose (BC)/nisin films with improved antibacterial activity and mechanical properties were successfully produced. The findings demonstrated that increased nisin production is associated with an upregulation of gene expression. Furthermore, results from Scanning electronic microscopy (SEM), Fourier transform infrared (FTIR), X-ray diffraction (XRD), and Thermogravimetric analysis (TG) confirmed the integration of nisin within BC. While being biocompatible with human cells, the BC/nisin composites exhibited antimicrobial activity. Moreover, mechanical property analyses showed a noticeable improvement in Young's modulus, tensile strength, and elongation at break by 161, 271, and 195 %, respectively. Additionally, the nisin content in fermentation broth was improved by 170 % after co-culture, accompanied by an 8 % increase in pH as well as 10 % decrease in lactate concentration. Real-time reverse transcription PCR analysis revealed an upregulation of 11 nisin-related genes after co-cultivation, with the highest increase in nisA (5.76-fold). To our knowledge, this is the first study which demonstrates that an increase in secondary metabolites after co-culturing is modulated by gene expression. This research offers a cost-effective approach for BC composite production and presents a technique to enhance metabolite concentration through the regulation of relevant genes. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638813 | ISSN: | 01418130 | DOI: | 10.1016/j.ijbiomac.2023.128977 |
顯示於: | 生物科技研究所 |
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