https://scholars.lib.ntu.edu.tw/handle/123456789/638876
標題: | The 2D Membrane Technology for Fabrication of 2D Stem Cell Culture System | 作者: | NAI-CHEN CHENG Huang, Wen Yen SUNG-JAN LIN MIN-HUEY CHEN Lee, I. Chi Shen, Chia Ning Li, Yi Chen Ethan |
公開日期: | 1-一月-2023 | 來源出版物: | Stem Cell-based Biosystems | 摘要: | This chapter introduces the thin films as the in vitro 2D culture stem cell culture systems. The fabrication process of 2D culture systems usually has no complex manufacturing procedure and offers user-friendly and easily operated protocols for everyone working with cell culture- or biology-related research in laboratories. The principle of surface modification of culture dishes or wells is paving thin films or thick membranes on the tissue culture surface of the flat plane through physical or chemical methods. Compared with the thin films, thick membranes used for culturing cells are usually fabricated through manufacturing technology to obtain the membrane-type substrates, which are cut and placed in culture dishes/wells as the surface for cell adhesion and growth. The possible mechanism pathways provide clear information to fabricate the thick membrane with specific microstructures to design the tissue-like cell culture microenvironments. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638876 | ISBN: | 9781003822684 | DOI: | 10.1201/9781003130048-5 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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