https://scholars.lib.ntu.edu.tw/handle/123456789/639089
標題: | Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice | 作者: | Tsai, Yi-Ting Ruan, Jhen-Wei Chang, Cherng-Shyang MEI-LAN KO Chou, Hsiu-Chuan Lin, Chi-Chien Lin, Chiao-Mei Huang, Chih-Ting Wei, Yu-Shan Liao, En-Chi Chen, Hsin-Yi Kao, Cheng-Yuan Chan, Hong-Lin |
關鍵字: | Anti-obesity; Antrodia cinnamomea; Hyperphagia; Intestinal Barrier; Leptin-deficient (ob/ob) mice | 公開日期: | 10-三月-2020 | 卷: | 12 | 期: | 3 | 來源出版物: | Nutrients | 摘要: | Obesity is associated with metabolic disorders. Thus, obesity prevention and treatment are essential for health. Antrodia cinnamomea (AC) is a multifunctional medicinal fungus used for the treatment of various diseases and for preventing diet-induced obesity. Leptin deficiency causes over-eating and spontaneous obesity. The concomitant metabolic symptoms are more severe than diet-induced obesity. Here, we used leptin-deficient (ob/ob) mice as an animal model for over-feeding to study the effect of AC on obesity. We fed C57BL/6 mice (WT, ob+/+) and ob/ob mice with AC for four weeks before performing qRT-PCR and immunoblot analysis to elaborate AC-modulated mechanisms. Further, we used Caco-2 cells as a human intestinal epithelial barrier model to examine the effect of AC on intestinal permeability. Our results suggested that AC reduces lipid deposits of the liver and epididymal white adipose tissue (EWAT) by promoting lipid metabolism and inhibiting lipogenesis-associated genes and proteins in ob/ob mice. Moreover, AC effectively repaired intestinal-barrier injury caused by leptin deficiency and enhanced intestinal barrier integrity in Caco-2 cells. Interestingly, AC significantly reduced body weight and EWAT with no compromise on food intake in ob/ob mice. Thus, AC effectively reduced obesity caused by leptin-deficiency and can potentially be used as a nutraceutical for treating obesity. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/639089 | ISSN: | 2072-6643 | DOI: | 10.3390/nu12030726 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。