https://scholars.lib.ntu.edu.tw/handle/123456789/640212
標題: | COP1-ERF1-SCE1 regulatory module fine-tunes stress response under light-dark cycle in Arabidopsis | 作者: | Lin, Wen-Chi Chang, Hui-Hsien Huang, Zi-Bin Huang, Lin-Chen Kuo, Wen-Chieh MEI-CHUN CHENG |
關鍵字: | COP1; ERF1; SCE1; drought; salt stress; sumoylation; ubiquitination | 公開日期: | 11-二月-2024 | 出版社: | John Wiley and Sons Inc | 卷: | Online Version of Record before inclusion in an issue | 來源出版物: | Plant, cell & environment | 摘要: | ETHYLENE RESPONSE FACTOR 1 (ERF1) plays an important role in integrating hormone crosstalk and stress responses. Previous studies have shown that ERF1 is unstable in the dark and its degradation is mediated by UBIQUITIN-CONJUGATING ENZYME 18. However, whether there are other enzymes regulating ERF1's stability remains unclear. Here, we use various in vitro and in vivo biochemical, genetic and stress-tolerance tests to demonstrate that both CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) and SUMO-CONJUGATING ENZYME 1 (SCE1) regulate the stability of ERF1. We also performed transcriptomic analyses to understand their common regulatory pathways. We show that COP1 mediates ERF1 ubiquitination in the dark while SCE1 mediates ERF1 sumoylation in the light. ERF1 stability is positively regulated by SCE1 and negatively regulated by COP1. Upon abiotic stress, SCE1 plays a positive role in stress defence by regulating the expression of ERF1's downstream stress-responsive genes, whereas COP1 plays a negative role in stress response. Moreover, ERF1 also promotes photomorphogenesis and the expression of light-responsive genes. Our study reveals the molecular mechanism of how COP1 and SCE1 counteract to regulate ERF1's stability and light-stress signalling crosstalk. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/640212 | ISSN: | 01407791 | DOI: | 10.1111/pce.14850 |
顯示於: | 生化科技學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。