https://scholars.lib.ntu.edu.tw/handle/123456789/640561
標題: | Development of a rapid aptamer-chemiluminescence sensor for detecting glyphosate pesticide residue in soybeans | 作者: | Weng, Rui Cian Tsou, Min Cheng Lee, Jyun Lin Tseng, Chao Ming Huang, Yu Fen Xiao, Yu Lin Lu, Yen Pei Chou, Wei Chun RUEY-FENG CHANG Chuang, Chun Yu |
關鍵字: | Aptamer | AuNPs | Chemiluminescence | Glyphosate | Soybean | 公開日期: | 15-五月-2024 | 卷: | 272 | 來源出版物: | Talanta | 摘要: | Glyphosate (GLY) is a widely used herbicide worldwide, particularly in cultivating genetically modified soybeans resistant to GLY. However, routine multi-residue analysis does not include GLY due to the complexity of soybean matrix components that can interfere with the analysis. This study presented the development of an aptamer-based chemiluminescence (Apt-CL) sensor for rapidly screening GLY pesticide residue in soybeans. The GLY-binding aptamer (GBA) was developed to bind to GLY specifically, and the remaining unbound aptamers were adsorbed onto gold nanoparticles (AuNPs). The signal was in the form of luminol-H2O2 emission, catalyzed by the aggregation of AuNPs in a chemiluminescent reaction arising from the GLY-GBA complex. The outcomes demonstrated a robust linear relationship between the CL intensity of GLY-GBA and the GLY concentration. In the specificity test of the GBA, only GLY and Profenofos were distinguished among the fifteen tested pesticides. Furthermore, the Apt-CL sensor was conducted to determine GLY residue in organic soybeans immersed in GLY as a real sample, and an optimal linear concentration range for detection after extraction was found to be between 0.001 and 10 mg/L. The Apt-CL sensor exploits the feasibility of real-time pesticide screening in food safety. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/640561 | ISSN: | 00399140 | DOI: | 10.1016/j.talanta.2024.125741 |
顯示於: | 資訊工程學系 |
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