Application of bulk segregant RNA-Seq (BSR-Seq) and allele-specific primers to study soybean powdery mildew resistance
Journal
BMC plant biology
Journal Volume
24
Journal Issue
1
Date Issued
2024-03-01
Author(s)
Abstract
Powdery mildew (PM) is one of the important soybean diseases, and host resistance could practically contribute to soybean PM management. To date, only the Rmd locus on chromosome (Chr) 16 was identified through traditional QTL mapping and GWAS, and it remains unclear if the bulk segregant RNA-Seq (BSR-Seq) methodology is feasible to explore additional PM resistance that might exist in other varieties.
Subjects
Bulk segregant RNA-Seq; MLO gene; Powdery mildew; QTL mapping; Resistance; Soybean; Transcriptome
Type
journal article