https://scholars.lib.ntu.edu.tw/handle/123456789/641433
標題: | Attenuation of diabetes-mediated muscle atrophy in rats by fish oil enriched omega-3 polyunsaturated fatty acids supplementation | 作者: | SHING-HWA LIU Lin, Wei Hsuan Tzeng, Huei Ping Chiang, Meng Tsan |
關鍵字: | Diabetes mellitus | Fish oil | Muscle atrophy | Omega-3 polyunsaturated fatty acids | Sarcopenia | 公開日期: | 1-一月-2023 | 出版社: | Taiwan Food and Drug Administration | 卷: | 31 | 期: | 3 | 起(迄)頁: | 458-472 | 來源出版物: | Journal of Food and Drug Analysis | 摘要: | Diabetes is associated with an increased risk of muscle wasting/atrophy, which adversely affects quality of life. We hypothesized that long term supplementation of fish oil may have protective effects against sarcopenia or muscle atrophy in streptozotocin (STZ) and high-fat (HF) diet-induced diabetic rat model. Wistar rats at age of 7 weeks were injected with saline or STZ to induce hyperglycemia. After one week, they were fed on a normal control diet or HF diet with/without supplementation of fish oil for 18 weeks. Feeding diabetic rats with a fish oil-enriched diet alleviated body weight loss and the impaired glucose tolerance using OGTT test. Although fish oil did not improve the decreased muscle mass, the muscle atrophy induced by diabetes was attenuated by fish oil in gastrocnemius, soleus, tibialis anterior, and extensor digitorum longus muscles. Fish oil supplementation reversed the decreased expression of phospho (p)-AKT, pmTOR, and p-p70s6k, which are molecules related to protein synthesis. Besides, protein degradation-related signaling pathways were inhibited by fish oil, such as increasing p-FoxO1 and decreasing Atrogin-1 and MURF1 protein expression. Fish oil down-regulated the expression of autophagy-related molecules including ATG5, p62, and LC3B II/I ratio, which may result in less muscle atrophy. Inflammation-related signaling regulators including TNF-a, NF-kB, AGEs, and RAGE were suppressed by fish oil supplementation as well. Moreover, the down-regulated p-AMPKa, SIRT1, and PGC-1 in diabetic rats were counteracted by fish oil, which may improve mitochondrial function and further block FoxO action. These data suggest that long-term fish oil supplementation exerts protective effects against diabetesinduced muscle atrophy, which may in turn ameliorate insulin resistance and impaired glucose tolerance. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/641433 | ISSN: | 10219498 | DOI: | 10.38212/2224-6614.3468 |
顯示於: | 毒理學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。