https://scholars.lib.ntu.edu.tw/handle/123456789/641496
標題: | Correlations between histological characterizations and methylation statuses of tumour suppressor genes in Wilms' tumours | 作者: | Lai, Yen-Chein MENG-YAO LU Wang, Wen-Chung Hou, Tai-Cheng Kuo, Chen-Yun |
關鍵字: | Wilms' tumour; methylation detection; molecular pathology; multiplex ligation-dependent probe amplification; nephroblastoma; tumour suppressor gene | 公開日期: | 六月-2022 | 卷: | 103 | 期: | 3 | 來源出版物: | International journal of experimental pathology | 摘要: | Wilms' tumour is a solid tumour that frequently occurs in children. Genetic changes in WT1 and epigenetic aberrations that affect imprinted control region 1 in WT2 loci are implicated in its aetiology. Moreover, tumour suppressor genes are frequently silenced by methylation in this tumour. In the present study, we analysed the methylation statuses of promoter regions of 24 tumour suppressor genes using a methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA)-based approach in 6 Wilms' tumours. Methylation of RASSF1 was specific to all 6 Wilms' tumours and was not observed in normal tissues. Moreover, methylated HIC1 was identified in stromal-type Wilms' tumours and methylated BRCA1 was identified in epithelial-type Wilms' tumours. Unmethylated CASP8, RARB, MLH1_167, APC and CDKN2A were found only in blastemal predominant-type Wilms' tumour. Our results indicated that methylation of RASSF1 may be a vital event in the tumorigenesis of Wilms' tumour, which informs its clinical and therapeutic management. In addition, mixed-type Wilms' tumours may be classified according to epithelial, stromal and blastemal components via MS-MLPA-based approach. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/641496 | ISSN: | 09599673 | DOI: | 10.1111/iep.12442 |
顯示於: | 醫學系 |
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