https://scholars.lib.ntu.edu.tw/handle/123456789/641651
標題: | The temporospatial relationship between mouse dental pulp stem cells and tooth innervation | 作者: | Chang, Shu Ya Chen, Rung Shu JULIA YU-FONG CHANG MIN-HUEY CHEN |
關鍵字: | Dental pulp stem cells | Human tooth development | Immunofluorescence staining | Mouse tooth development | Nerve of pulp | 公開日期: | 1-一月-2024 | 來源出版物: | Journal of Dental Sciences | 摘要: | Background: Dental pulp stem cells (DPSCs) exhibit versatile differentiation capabilities, including neural differentiation, prompting the hypothesis that they may be implicated in the neurodevelopment of teeth. This study aimed to explore the temporospatial dynamics between DPSCs and tooth innervation, employing immunofluorescence staining and fluorescent dye injections to investigate the distribution of DPSCs, neural stem cells (NSCs), nerve growth cones, and sensory nerves in developing mouse tooth germs at various stages. Materials and methods: Immunofluorescence staining targeting CD146, Nestin, and GAP-43, along with the injection of AM1-43 fluorescent dye, were utilized to observe the distribution of DPSCs, NSCs, nerve growth cones, and sensory nerves in mouse tooth germs at different developmental stages. Results: Positive CD146 immunostaining was observed in microvascular endothelial cells and pericytes within and around the tooth germ. The percentage of CD146-positive cells remained consistent between 4-day-old and 8-day-old second molar tooth germs. Conversely, Nestin expression in odontoblasts and their processes decreased in 8-day-old tooth germs compared to 4-day-old ones. Positive immunostaining for GAP-43 and AM1-43 fluorescence revealed the entry of nerve growth cones and sensory nerves into the pulp in 8-day-old tooth germs, while these elements were confined to the dental follicle in 4-day-old germs. No co-localization of CD146-positive DPSCs with nerve growth cones and sensory nerves was observed. Conclusion: DPSCs and NSCs were present in dental pulp tissue before nerves penetrated the pulp. The decline in NSCs after nerve entry suggests a potential role for DPSCs and NSCs in attracting neural growth and/or differentiation within the pulp. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/641651 | ISSN: | 19917902 | DOI: | 10.1016/j.jds.2024.02.007 |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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