https://scholars.lib.ntu.edu.tw/handle/123456789/641706
標題: | A rapid DNA amplification assay for cetacean product identification: Providing insights for illegal cetacean utilization and implications for conservation | 作者: | Ho, Yun Chan, Kun Wei WEI-CHENG YANG |
關鍵字: | Bioassay | Cetacean | Conservation | Human health | Recombinase polymerase amplification | Resource management | 公開日期: | 1-五月-2024 | 卷: | 251 | 來源出版物: | Ocean and Coastal Management | 摘要: | Illegal utilization of cetaceans was continuously reported worldwide, posing potential threat to the sustainability of cetacean species. In addition, public health is at risk with the consumption of cetacean-based food products due to high contamination level and zoonotic pathogens. However, the scale of cetacean exploitation remains unclear. This study presented a portable, rapid, and sensitive recombinase polymerase amplification (RPA) assay coupled with dual-zone lateral flow strip, that can achieve identification of cetaceans within 30 min. In addition, this assay could differentiate baleen whales and toothed whales. Unlike conventional immunochromatographic lateral flow test, this assay is compatible with cooked and digested sample types. With this assay, inspecting suspicious cetacean products could become more efficient. Moreover, it can improve the understanding of the pathway of illegal trade and consumption of cetacean products. A more comprehensive insight of the cetacean utilization is essential for establishing feasible management policy, especially toward certain vulnerable and endangered species. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85186961616&doi=10.1016%2fj.ocecoaman.2024.107084&partnerID=40&md5=0d8eb8163f024746943d7c9d29101601 https://scholars.lib.ntu.edu.tw/handle/123456789/641706 |
ISSN: | 09645691 | DOI: | 10.1016/j.ocecoaman.2024.107084 |
顯示於: | 獸醫學系 |
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