Electrospun fibers as a solid-state real-time zinc ion sensor with high sensitivity and cell medium compatibility
Journal
Advanced Functional Materials
Journal Volume
23
Journal Issue
12
Date Issued
2013-03-25
Author(s)
Syu, Jia Hao
Cheng, Yi Kai
Hong, Wun Yan
Wang, Hsing Ping
Lin, Yu Chao
Meng, Hsin Fei
Zan, Hsiao Wen
Horng, Sheng Fu
Chang, Gao Fong
Hung, Chen Hsiung
Chiu, Yu Cheng
Tsai, May Jywan
Cheng, Henrich
Abstract
meso-2,6-Dichlorophenyltripyrrinone (TPN-Cl2), a probe molecule for zinc II ions, is dispersed in a polymer host. The red fluorescence peak at 620 nm appears when the molecule forms a complex with zinc at its center. TPN-Cl2 has a high selectivity for zinc II and tolerates many common metal ions present in the human body. The probe molecules are blended with a hydrogel polymer, poly(2-hydroxyethyl methacrylate) (poly HEMA), with 30 wt% dimethylformamide (DMF). The fiber structure with 1 μm diameter is made by electrospinning in DMF solution of the probe and poly HEMA mixture. The fibrous film detects zinc ions with concentrations as low as 10-6 M in real-time both in water and in the commonly used cell culture liquid media Dulbecco's modified Eagle medium (DMEM) and fetal bovine serum (FBS), which contain many metal ions and proteins. The time-resolution is 5 min for 10 -6 M and 1 min for 10-5 M. This sensitivity and response speed satisfy the requirements for non-invasive biomedical studies. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Subjects
electrospun fibers | real-time detection | solid-state materials | zinc ion sensors
SDGs
Type
journal article