DC Field | Value | Language |
dc.contributor.author | 謝學真 | zh_TW |
dc.creator | 謝學真 | - |
dc.date | 2005 | zh_TW |
dc.date.accessioned | 2006-07-25T09:39:55Z | - |
dc.date.accessioned | 2018-06-28T17:28:04Z | - |
dc.date.available | 2006-07-25T09:39:55Z | - |
dc.date.available | 2018-06-28T17:28:04Z | - |
dc.date.issued | 2005 | - |
dc.identifier | 932321B002034 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/9435 | - |
dc.description.abstract | 血管壁的內皮細胞(ECs)持續受到血
液流動所產生的剪力刺激,穩定的剪力能保
護內皮細胞並抑制發炎反應相關的基因表
現。本研究探討在發炎反應中重要的細胞素interferon-gamma (IFN-γ)刺激下,剪力是否
對內皮細胞具有保護效果。採用牛的動脈內
皮細胞(BAEC)和人的臍帶靜脈內皮細胞
(HUVEC)為實驗對象,觀察內皮細胞受到
剪力(25 dyn/cm 2 )作用時,細胞中訊息調控
及基因表現的改變。以細胞素IFN摯瑬敳獩 (2.5
ng/ml)刺激內皮細胞會活化JAK1/2 及
STAT1 ,造成JAK1/2 及STAT1 的酪氨酸
(Tyr 701)、絲氨酸(Ser727)磷酸化增加。Tyr
701 磷酸化在30 分鐘達到最高點,而Ser727
磷酸化在30 分鐘後才開始明顯上升。以不
同濃度的IFN摯瑬敳獩 刺激內皮細胞30 分鐘,Tyr
701 、Ser727 磷酸化程度會隨著IFN摯瑬敳獩 濃度的
增加而上升。然而,剪力會部分抑制IFN摯瑬敳獩
所引發的JAK1/2 及Tyr 701 磷酸化,隨著
剪力施予的時間增加,抑制的效果也越明
顯。而隨著剪力大小的增加,抑制IFN摯瑬敳獩 引
發的Tyr 701 磷酸化,其效果也越明顯,但
並不會影響Ser727 磷酸化的程度。由於
IFN摯瑬敳獩 透過活化STAT1 ,可引發內皮細胞中
化學趨化蛋白IP-10 的表現,本研究後續將
探討剪力對IFN摯瑬敳獩 所引發的下游基因(包括
IP-10 等)表現之影響。綜合上述,剪力可藉抑制發炎反應中重要的細胞素IFN摯瑬敳獩 所引發
的JAK1/2 及STAT1 Tyr701 磷酸化,進而
對內皮細胞產生保護效果。 | zh_TW |
dc.description.abstract | Endothelial cells (ECs) are constantly exposed
to blood flow-induced shear stress. Laminar shear
stress protects ECs from endothelial dysfunction.
In the present study, the effect of shear stress (SS)
on IFN摯瑬敳獩 -induced responses including STAT1 and
JAK1/2 phosphorylation in ECs was examined.
SS had no effect on Tyr701 and Ser727
phosphorylation of STAT1 in ECs under basal
condition. Further, IFN摯瑬敳獩 triggered the activation
of JAK/STAT1 signaling pathway with the
phosphorylation of JAK1/2 and phosphorylation
of Tyr701 and Ser727 in STAT1. Under
IFN摯瑬敳獩 treatment, Tyr-701 phosphorylation
approached the highest level at 30 minutes and
Ser-727 phosphorylation obviously rised after 30
minutes. IFN摯瑬敳獩 induced Tyr-701 and Ser-727
phosphorylation in a dose-dependent manner,
which Tyr-701 and Ser-727 phosphorylation
approached their highest levels with IFN摯瑬敳獩
concentration of 2.5 ng/ml. When SS was applied
to IFN摯瑬敳獩 -treated ECs, it significantly inhibited the
IFN摯瑬敳獩 -induced Tyr701 phosphorylation of STAT1
in a shear force- and time course-dependent
manner whiles Ser727 phosphorylation was unaffected. IFN摯瑬敳獩 , through STAT1 signaling
pathway, induced downstream chemokine IP-10
expression. Whether SS affects IFN摯瑬敳獩 -induced
downstream target gene expressions will be
examined in the future study. To sum up, our
results demonstrated that SS attenuates
IFN摯瑬敳獩 -induced JAK1/2 and STAT1 activation that
will provide a protective effect on ECs during
inflammatory response. | en |
dc.format | application/pdf | zh_TW |
dc.format.extent | 1526041 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學化學工程學系暨研究所 | zh_TW |
dc.rights | 國立臺灣大學化學工程學系暨研究所 | zh_TW |
dc.subject | 剪力 | zh_TW |
dc.subject | 內皮細胞 | zh_TW |
dc.subject | 細胞素 | zh_TW |
dc.subject | 化學趨化蛋白 | zh_TW |
dc.subject | shear stress | en |
dc.subject | endothelial cells | en |
dc.subject | cytokine | en |
dc.subject | chemokine | en |
dc.title | 血管內皮細胞發炎反應的細胞與分子機制─在發炎物刺激下血管內皮細胞中剪流所扮演之保護角色: 剪流對干擾素引發Stat1訊息傳導及相關基因表現之調控(1/3) | zh_TW |
dc.title | Protective role of shear flow in inflammatory agent-stimulated vascular endothelial cells: Regulation of interferon-induced Stat1 signaling and related gene expression by shear flow(1/3) | en |
dc.type | report | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/9435/1/932321B002034.pdf | - |
dc.coverage | 計畫年度:93;起迄日期:2004-08-01/2005-10-31 | zh_TW |
item.openairecristype | http://purl.org/coar/resource_type/c_93fc | - |
item.openairetype | report | - |
item.languageiso639-1 | zh_TW | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
crisitem.author.dept | Chemical Engineering | - |
crisitem.author.orcid | 0000-0003-1231-6670 | - |
crisitem.author.parentorg | College of Engineering | - |
Appears in Collections: | 化學工程學系
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