CuO NWs boosted triboelectric microfluidic nanosensor functionalized by collagen-protein interactions for real-time platelet count monitoring
Journal
Chemical Engineering Journal
Journal Volume
490
ISSN
1385-8947
Date Issued
2024-06-15
Author(s)
Jia-Cheng Lin
Kuldeep Kaswan
Subhodeep Chatterjee
Yu-Wen Wu
Manish Kumar Sharma
Ashok Ranjan
Snigdha Roy Barman
Yu-Zih Lin
Thierry Burnouf
Wilfrid Boireau
Ming-Yen Lu
Yong-Kwang Tu
I-Chang Su
Ping-Hsiu Wu
Yu-Jui Fan
DOI
10.1016/j.cej.2024.151586
Abstract
Blood platelet count significantly affects the development of severe conditions like myocardial infarction, peripheral arterial ischemia, respiratory compromise, stroke, diabetes, coronavirus disease 2019 (COVID-19), along with chemotherapy patients and those suffering cardiovascular diseases (CVDs). These conditions necessitate frequent monitoring of platelet counts to guide diagnostic and therapeutic decisions. However, existing techniques are relatively time-consuming, lack of accuracy and require precise operation. The emergence of these severe diseases underscored the need to develop advanced platelet count-monitoring techniques which are rapid, highly precise, and conveniently portable for point-of-care applications. In this study, we emphasized the development of a triboelectric microfluidic nanosensor (TMNS) for platelet quantification through the assessment of flow resistance. The functionality of TMNS device is based on immobilization of platelets on a collagen layer coated inside a microfluidic channel. The triboelectric voltage output is measured as a detection signal of the flow resistance and is enhanced by incorporating high surface area copper oxide nanowires (CuO NWs) on the interior of copper tubes. These copper tubes serve as terminal electrodes and for flow guiding. The flow resistance of plasma solutions is elevated when the platelet concentration increases due to heightened adherence of platelets onto the collagen layer. Variations in flow resistance induce alterations in contact electrification, causing changes in output voltage at load terminals. Fine-tuning of the TMNS device was achieved by optimizing the channel width and length, flowing liquid viscosity, and voltage measurement technique. Platelet quantification sensing data were acquired through the combination of platelet-rich plasma (PRP) and platelet-poor plasma (PPP) solutions. The described device exhibits promising capabilities for platelet-count monitoring in whole-blood samples collected from three distinct patient groups, showcasing its potential impact in precise point-of-care applications.
Subjects
CuO NWs
Flow sensing
Microfluidics
Platelet counts
Platelet-collagen binding
Triboelectric nanosensor
Publisher
Elsevier BV
Type
journal article