In vitro intracellular studies of pH and thermo-triggered doxorubicin conjugated magnetic SBA-15 mesoporous nanocarriers for anticancer activity against hepatocellular carcinoma
Journal
Journal of Industrial and Engineering Chemistry
Series/Report No.
Journal of Industrial and Engineering Chemistry
Journal Volume
102
Start Page
1-16
ISSN
1226-086X
Date Issued
2021-10-25
Author(s)
Ndumiso Vukile Mdlovu
Kuen-Song Lin
Chi-Cheng Hsieh
You-Sheng Lin
Maria Janina Carrera Espinoza
Abstract
Hepatocellular carcinoma (HCC) is the most prevalent type of liver cancer, accounting for about 75% of all liver cancers. It is the third most common basis for cancer mortality worldwide, and unfortunately, its treatment is often limited by the shortage of appropriate therapeutic options and side effects caused by the current treatment methods. To overcome this, doxorubicin (DOX)-loaded pH-/thermo-responsive magnetic mesoporous nanocarriers were formulated and evaluated for their in vitro anticancer activity against HCC. These nanocarriers consist of iron oxide (IO) nanoparticles conjugated with SBA-15 (S15) and Pluronic F127 (PF) to form IOS15 nanocomposites and IOS15@PF nanocarriers. The prepared nanocarriers were superparamagnetic with saturation magnetizations of IOS15 and IOS15@PF being 76.3 and 72.1 emu/g, respectively. Small-angle neutron/X-ray scattering (SANS/SAXS) studies showed that the developed nanocarriers are temperature-sensitive and possess hexagonally arranged structures. Cell viability studies demonstrated that IOS15@PF@DOX nanocomplexes induced more apoptosis or necrosis. A temperature (69% release after 48 h)- and pH (70% release after 48 h)-dependent DOX release was observed, whereby more DOX was released at a high temperature of 42 °C and pH value of 5.4. Thus, the developed nanocarriers possess great potential for use in the targeted delivery of conventional chemotherapeutic drugs with enhanced efficiency.
SDGs
Publisher
Elsevier BV
Type
journal article