One-step synthesis of fluorescein modified nano-carbon for Pd(ii) detection via fluorescence quenching
Journal
Analyst
Journal Volume
137
Journal Issue
9
Start Page
2054
End Page
2062
ISSN
00032654
Date Issued
2012
Author(s)
Abstract
Carbon black (CB) nanoparticles modified with fluorescein, a highly fluorescent molecule, were prepared using a facile and efficient methodology. Simply stirring CB in aqueous solution containing fluorescein resulted in the strong physisorption of fluorescein onto the CB surface. The resulting Fluorescein/CB was then characterised by means of X-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV), fluorescence microscopy and fluorescence spectroscopy. The optimum experimental conditions for fluorescence of Fluorescein/CB viz. fluorescence excitation and emission wavelengths, O 2 removal and the amount of Fluorescein/CB used, were investigated. The Fluorescein/CB was used as a fluorescent probe for the sensitive detection of Pd(ii) in water, based on fluorescence quenching. The results demonstrated that the fluorescence intensity of Fluorescein/CB decreased with increasing Pd(ii) concentration, and the fluorescence quenching process could be described by the Stern-Volmer equation. The limit of detection (LOD) for the fluorescence quenching of Fluorescein/CB by Pd(ii) in aqueous solution was found to be 1.07 μM (based on 3σ). Last, approaches were studied for the removal of Fe(iii) which interferes with the fluorescence quenching of Fluorescein/CB. Complexation of Fe(iii) with salicylic acid was used to enhance and control the selectivity of Fluorescein/CB sensor towards Pd(ii) in the presence of Fe(iii). © 2012 The Royal Society of Chemistry.
SDGs
Publisher
Royal Society of Chemistry
Type
journal article