Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator
Journal
Nature Plants
Journal Volume
9
Journal Issue
2
Start Page
355
End Page
371
ISSN
20550278
Date Issued
2023-01-12
Author(s)
Wang P.
Zhao X.
Arora D.
Wang R.
Eeckhout D.
Van Leene J.
Kumar R.
Houbaert A.
De Winne N.
Mylle E.
Vandorpe M.
Korver R.A.
Testerink C.
Gevaert K.
Vanneste S.
De Jaeger G.
Van Damme D.
Russinova E.
Abstract
Adaptor protein (AP) complexes are evolutionarily conserved vesicle transport regulators that recruit coat proteins, membrane cargoes and coated vesicle accessory proteins. As in plants endocytic and post-Golgi trafficking intersect at the trans-Golgi network, unique mechanisms for sorting cargoes of overlapping vesicular routes are anticipated. The plant AP complexes are part of the sorting machinery, but despite some functional information, their cargoes, accessory proteins and regulation remain largely unknown. Here, by means of various proteomics approaches, we generated the overall interactome of the five AP and the TPLATE complexes in Arabidopsis thaliana. The interactome converged on a number of hub proteins, including the thus far unknown adaptin binding-like protein, designated P34. P34 interacted with the clathrin-associated AP complexes, controlled their stability and, subsequently, influenced clathrin-mediated endocytosis and various post-Golgi trafficking routes. Altogether, the AP interactome network offers substantial resources for further discoveries of unknown endomembrane trafficking regulators in plant cells. © 2023, The Author(s), under exclusive licence to Springer Nature Limited.
Publisher
Nature Research
Type
journal article