A rapid spectroscopic platform in identifying cisplatin resistance in head and neck squamous cell carcinoma
Journal
Imaging, Therapeutics, and Advanced Technology in Head and Neck Surgery and Otolaryngology 2024
Journal Volume
12818
Start Page
1281804
ISSN
16057422
Date Issued
2024-03-13
Author(s)
Editor(s)
Brian J. F. Wong
Justus F. Ilgner
Abstract
At present, the administration of chemotherapy drugs is largely based on physician experience and clinical guidelines. Clearly, a personalized solution would significantly benefit patients in the improvement of clinical outcomes. In this work, we used a spectroscopic approach in which a water-soluble tetrazolium salt (WST-8) assay was applied to patient-derived slice organoids. WST-8 would react with intracellular dehydrogenase resulting in the formation of formazan dye. The absorption at 450nm can be used to assess the viability of the organoids 5 days after initiation of organoid culture. With additional development, this approach may be used to improve the clinical outcome of patients undergoing chemotherapy.
Publisher
SPIE
Type
conference paper