Antimicrobial Peptides Display Strong Synergy with Vancomycin Against Vancomycin-Resistant , , and Wild-Type .
Journal
International journal of molecular sciences
Journal Volume
21
Journal Issue
13
Start Page
4578
End Page
1-12
ISSN
1422-0067
Date Issued
2020-06-27
Author(s)
Abstract
There is an urgent and imminent need to develop new antimicrobials to fight against antibiotic-resistant bacterial and fungal strains. In this study, a checkerboard method was used to evaluate the synergistic effects of the antimicrobial peptide P-113 and its bulky non-nature amino acid substituted derivatives with vancomycin against vancomycin-resistant , and wild-type . Boron-dipyrro-methene (BODIPY) labeled vancomycin was used to characterize the interactions between the peptides, vancomycin, and bacterial strains. Moreover, neutralization of antibiotic-induced releasing of lipopolysaccharide (LPS) from by the peptides was obtained. Among these peptides, Bip-P-113 demonstrated the best minimal inhibitory concentrations (MICs), antibiotics synergism, bacterial membrane permeabilization, and supernatant LPS neutralizing activities against the bacteria studied. These results could help in developing antimicrobial peptides that have synergistic activity with large size glycopeptides such as vancomycin in therapeutic applications.
Subjects
antibiotic resistance
antimicrobial peptide
bulky non-nature amino acid
synergism
vancomycin
SDGs
Type
journal article