Immunopathological effects of Agaricus blazei Murill polysaccharides against Schistosoma mansoni infection by Th1 and NK1 cells differentiation
Journal
International Immunopharmacology
Journal Volume
73
Start Page
502-514
ISSN
1567-5769
Date Issued
2019-08
Author(s)
Lin, Ming-Hong
Lee, Kin-Mu
Peng, Shih-Yi
Lin, Ching-Nan
Chen, Chin-Chu
Fan, Chia-Kwung
Cheng, Po-Ching
Abstract
In this study, we examined the ability of A. blazei Murill polysaccharides (AB-PS) to activate the immune system in vivo and the protective activity exhibited against parasitic S. mansoni in the murine model. AB-PS treatment significantly reduced the worm and egg burden in infected BALB/c and C57BL/6 mice with dose- and time-dependent manners. Additionally, a dose- and time-dependent expression of IL-2, INF-γ, and TNF-α cytokines was also observed in both strains of mice treatments. Using T1/T2 doubly transgenic mice, we demonstrated that AB-PS-treated mice splenocytes initiated early differentiation of Th1 and NK1 cells, which was consistent with the reduction course of Schistosoma infection. Although AB-PS treatment enhanced the Th1 response, it did not suppress Th2 cell activity in treated mice. Histopathological data of the livers showed AB-PS treatment significantly attenuated the liver fibrosis induced by S. mansoni eggs. AB-PS augmented type-1 responses by inducing Th1 and NK1 cell differentiation to effectively decrease the infection rate of S. mansoni. Furthermore, AB-PS treatment may not only inhibit the schistosome infection, but also improving the pathological effects of granulomas formation. This study provides evidence for a novel therapeutic potential, by which A. blazei Murill may be used to treat or prevent schistosome infection.
Subjects
Agaricus blazei Murill polysaccharides
Immunomodulation
NK cell
Schistosoma mansoni
T1/T2 doubly transgenic mice
SDGs
Publisher
Elsevier BV
Type
journal article