Immunolocalization Analysis of C4 Proteins in the Leaf Tissue of Rice
Journal
Methods in Molecular Biology
Part Of
Rice Genome Engineering and Gene Editing
Journal Volume
2238
Start Page
285
End Page
292
ISSN
1064-3745
1940-6029
ISBN (of the container)
978-1-0716-1067-1
978-1-0716-1070-1
978-1-0716-1068-8
DOI (of the container)
https://doi.org/10.1007/978-1-0716-1068-8
Date Issued
2021
Author(s)
Abstract
Immunolocalization analysis is a principal tool to study protein expression and subcellular distribution in plant cells or tissues. In this chapter, we present the method of the preparation of lightly fixed fresh rice leaf tissue for immunolocalization analysis and detection of the protein of interest using fluorescent probes by fluorescent microscopy. This method especially does not need the process of embedding plant materials that saves time and prevents alterations of cellular compounds and structure during sample preparation. Using this method, the C4 rice project compared the expressions of the proteins of interest among C4 model plants, wild-type rice, and transgenic or mutant plants and successfully selected the transgenic plants with the correct location of each protein to create a C4 rice prototype.
Subjects
ACP5
C4 proteins
C4 rice project
Confocal microscopy
GFP
Immunolocalization
OAA
PCK
PEPC
PPDK
TEM
Publisher
Springer
Type
book part