探討一全新基因對於線蟲中siRNA精子活化路徑之影響
Other Title
Exploring Novel Genes in Small RNA
Mediated Activation Pathways for C. elegans Sperm Function
Mediated Activation Pathways for C. elegans Sperm Function
Journal
2025臺大學士論文獎
Date Issued
2025
Author(s)
Chen-Hsi Chang
Advisor
Abstract
This study focuses on the role of a small RNA activated pathway, the alg 3/4 pathway, in C. elegans sperm activation. In this pathway, 26G RNAs, which are associated with ALG 3/4, enhance the expression of proteins essential for spermatogenesis. A key protein in this pathway, NYN 3 , is necessary for the production of 26G RNA. When mutated, NYN 3 disrupt s 26G RNA biogenesis, leading to sterility
at 25°C. Interestingly, we identified a gene, mutA, that partially rescues the fertility defect caused by the NYN 3 mutation by restoring some aspects of gene regulation.
Previous research has largely demonstrated that small RNAs, especially in various organisms, are involved in gene silencing. However, in both mammals and C. elegans , certain small RNA pathways have been shown to participate in translational and transcriptional activation, especially in processes r elated to sperm activation. In light of these findings, this study aims to further explore the role of the previously unknown gene mutA in C. elegans to uncover its function in sperm activation.
Using SNP analysis, we narrowed down the potential location of the mutA gene to two regions on chromosomes IV and V of C. elegans . Additionally, we performed whole genome sequencing ( W GS) to identify mutations within these regions. Ultimately, we identified Y38F2AR.10 in the IV, 7 region as a likely candidate for mu tA. To confirm this, we employed CRISPR Cas9 technology to generate a
Y38F2AR.10 mutant.
Our next steps involve determining the precise function of mutA and whether it affects the expression of spermatogenesis related proteins. We also aim to elucidate the role of mutA within the role of mutA within the algalg--3/4 3/4 pathway. By understanding these mechanisms, this pathway. By understanding these mechanisms, this research hopes to shed light on gene regulatory processes and uncover further insights research hopes to shed light on gene regulatory processes and uncover further insights into sperm development in mammals, which could ultimately lead to applications in into sperm development in mammals, which could ultimately lead to applications in addreaddressing human infertility issues.ssing human infertility issues.
Subjects
spermatogenesis
siRNA
alg 3/4 pathway
gene regulation
WGS sequencing
SNP analysis
Publisher
國立臺灣大學醫學系
Description
獎項：傅斯年獎；指導教授：蔡欣祐
Type
thesis
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