Convergent evolution of hexenal isomerases in Lepidoptera and plants
Journal
Nature Ecology & Evolution
ISSN
2397-334X
Date Issued
2026-02-27
Author(s)
Wu, Bulah Chia-hsiang
Sharaf, Abdoallah
Heijblom, Sophie Maartje Elisabeth
Prattis, Ilias
Tan, Ching-Wen
Schilder, Rudolf J.
Ali, Jared Gregory
Allmann, Silke
Abstract
Green leaf volatiles (GLVs) are six-carbon volatile organic compounds that mediate plant responses to environmental stresses. The quantity and composition of emitted GLVs can vary with stress type, allowing plants to fine-tune their volatile blends. In addition, insect herbivores are capable of modulating these emissions. A key mechanism underlying this plasticity is the conversion of Z-3-hexenal to E-2-hexenal by the enzyme (3Z):(2E)-hexenal isomerase (Hi), which reshapes GLV profiles and may influence multitrophic interactions. Here we investigate the evolutionary origin, functional diversification and catalytic mechanisms of lepidopteran Hi homologues, which belong to the glucose–methanol–choline oxidoreductase family. Phylogenetic analysis of 34 lepidopteran species identified a distinct glucose–methanol–choline-β subclade enriched in Hi homologues, largely confined to the Apoditrysia lineage. Functional assays showed species-specific variation in Hi activity, with Manduca sexta Hi-1 displaying the highest activity among tested homologues under identical protein concentrations, both in vitro and in planta. Structural modelling and site-directed mutagenesis revealed that Hi activity requires a flavin adenine dinucleotide cofactor enabling the identification of key residues critical for flavin adenine dinucleotide binding. Comparative phylogenetics further suggests that Hi enzymes in plants and Lepidoptera evolved independently from unrelated enzyme families, representing a case of functional convergence coinciding with the Cretaceous angiosperm radiation.
Subjects
Ecophysiology
Entomology
Molecular evolution
Plant evolution
Publisher
Springer Science and Business Media LLC
Type
journal article
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