Enhancing human-induced pluripotent stem cell proliferation and cardiac differentiation through 810-nm photobiomodulation
Journal
Lasers in Medical Science
Journal Volume
41
Journal Issue
1
Start Page
25
ISSN
1435-604X
02688921
Date Issued
2026-02-10
Author(s)
Abstract
Photobiomodulation (PBM) has been shown to enhance cell growth and differentiation. This study investigates the effects of 810-nm PBM on the proliferation and cardiac differentiation of human-induced pluripotent stem cells (hiPSCs), utilizing a custom-made LED device. This device is compatible with standard cell incubators and features an LED array that ensures uniform light exposure to cells in each well of a 96-well plate. hiPSCs were irradiated with 810-nm light at intensities of 1.0, 1.5, and 2.0 mW/cm² for 15 min daily. Outcomes assessed included cell viability, mitochondrial function and morphology, and cardiomyocyte differentiation. The results show that PBM at 1.5 mW/cm² enhanced hiPSC proliferation and cardiac differentiation, yielding a higher number of spontaneously beating cardiomyocytes compared to controls. These findings suggest that 810-nm PBM may improve the efficiency of cardiac cell generation for both research and therapeutic applications.
Subjects
810 nm LED
Differentiation
iPSC
Photobiomodulation
Publisher
Springer Science and Business Media LLC
Type
journal article