Printing Peptide Arrays with a Complementary Metal Oxide Semiconductor Chip
Part Of
Fundamentals and Application of New Bioproduction Systems
Journal Volume
137
Start Page
1
End Page
23
ISSN
0724-6145
1616-8542
ISBN (of the container)
9783642415210
9783642415210
Date Issued
2013-01-01
Author(s)
Loeffler, Felix F.
Muenster, Bastian
Striffler, Jakob
Liu, Fanny C.
Ralf Bischoff, F.
Doersam, Edgar
Breitling, Frank
Nesterov-Mueller, Alexander
Abstract
In this chapter, we discuss the state-of-the-art peptide array technologies, comparing the spot technique, lithographical methods, and microelectronic chip-based approaches. Based on this analysis, we describe a novel peptide array synthesis method with a microelectronic chip printer. By means of a complementary metal oxide semiconductor chip, charged bioparticles can be patterned on its surface. The bioparticles serve as vehicles to transfer molecule monomers to specific synthesis spots. Our chip offers 16,384 pixel electrodes on its surface with a spot-to-spot pitch of 100 μm. By switching the voltage of each pixel between 0 and 100 V separately, it is possible to generate arbitrary particle patterns for combinatorial molecule synthesis. Afterwards, the patterned chip surface serves as a printing head to transfer the particle pattern from its surface to a synthesis substrate. We conducted a series of proof-of-principle experiments to synthesize high-density peptide arrays. Our solid phase synthesis approach is based on the 9-fluorenylmethoxycarbonyl protection group strategy. After melting the particles, embedded monomers diffuse to the surface and participate in the coupling reaction to the surface. The method demonstrated herein can be easily extended to the synthesis of more complicated artificial molecules by using bioparticles with artificial molecular building blocks. The possibility of synthesizing artificial peptides was also shown in an experiment in which we patterned biotin particles in a high-density array format. These results open the road to the development of peptide-based functional modules for diverse applications in biotechnology.
Publisher
Springer Berlin Heidelberg
Type
book part