Prolonged cold exposure enhances regeneration potential in Arabidopsis
Journal
Developmental Cell
Journal Volume
61
Journal Issue
8
Start Page
1717
End Page
1729.e3
ISSN
1534-5807
Date Issued
2026-08-12
Author(s)
Hung, Fu-Yu
Ince, Yetkin Caka
Kawamura, Ayako
Takebayashi, Arika
Chen, Yu
Nagae, Takuya T.
Iwase, Akira
Takeda-Kamiya, Noriko
Toyooka, Kiminori
Shi, Dongbo
Moreno-Risueno, Miguel
Sugimoto, Keiko
Abstract
Prolonged cold exposure over winter impacts plant growth and development, but its role beyond flowering regulation remains underexplored. In this study, we show that extended cold enhances regenerative capacity, promoting both callus formation and shoot regeneration in Arabidopsis . This enhancement is mediated by the cold-induced AP2/ERF transcription factors C-REPEAT/DRE-BINDING FACTOR 1 (CBF1), CBF2, and CBF3, which interact with the histone acetyltransferase (HAT) HISTONE ACETYLTRANSFERASE OF THE GNAT FAMILY 1 (HAG1). The CBFs recruit HAG1 to the loci of key regeneration regulators, such as WUSCHEL-RELATED HOMEOBOX 5 ( WOX5 ), to promote their expression via histone acetylation. Our findings thus uncover an epigenetic mechanism by which prolonged cold primes plants for enhanced regeneration, highlighting how environmental cues influence developmental plasticity in plants.
Subjects
Arabidopsis
epigenetics
histone acetylation
plant regeneration
prolonged cold
Publisher
Elsevier BV
Type
journal article