https://scholars.lib.ntu.edu.tw/handle/123456789/89026
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ke, Meng-Wei | en |
dc.contributor.author | Jiang, Yan-Nian | en |
dc.contributor.author | Li, Yi-Hung | en |
dc.contributor.author | Tseng, Ting-Yu | en |
dc.contributor.author | Kung, Ming-Shung | en |
dc.contributor.author | CHIUN-SHENG HUANG | en |
dc.contributor.author | Cheng, Winston Teng-Kuei | en |
dc.contributor.author | Hsu, Jih-Tay | en |
dc.contributor.author | Ju, Yu-Ten | en |
dc.creator | Cheng, Winston Teng-Kuei;Kung, Ming-Shung;Tseng, Ting-Yu;Li, Yi-Hung;Jiang, Yan-Nian;Ke, Meng-Wei;Ju, Yu-Ten;Hsu, Jih-Tay;Huang, Chiun-Sheng | - |
dc.date.accessioned | 2012-01-30T10:26:40Z | - |
dc.date.accessioned | 2018-06-29T04:40:26Z | - |
dc.date.available | 2012-01-30T10:26:40Z | - |
dc.date.available | 2018-06-29T04:40:26Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1011-2367 | - |
dc.identifier.uri | http://www.scopus.com/inward/record.url?eid=2-s2.0-34249911216&partnerID=MN8TOARS | - |
dc.identifier.uri | http://scholars.lib.ntu.edu.tw/handle/123456789/329085 | - |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw/bitstream/246246/238490/-1/05.pdf | - |
dc.description.abstract | Caveolin-1 of the caveolin family of proteins regulates mammary gland development and has been shown to play a contradictory role in breast tumor progression. A specific anti-Caveolin-1 antibody will be useful for functional study of Caveolin-1 in different tissues. In this study, we generated a rabbit polyclonal antibody that specifically recognizes the N-terminal amino acids 50-65 of Caveolin-1. This polyclonal antibody specifically reacted with Caveolin-1 extracted from cells of different species, including human epithelial A431 cells, goat primary mammary epithelial cells and mice fibroblast NIH 3T3 cells, by Western blotting. Endogenous Caveolin-l protein expressing in cells and normal human tissues was detected by this polyclonal antibody using immunocytofluorescent and immunohistochemical staining, respectively. Furthermore, an apparent decrease in Caveolin-1 expression in tumorous breast and colon tissues was detected by this polyclonal antibody. In conclusion, we have identified amino acids 50-65 of Caveolin-1, which contains an epitope that is specific to Caveolin-l and is conserved in the human, goat and mouse. In future, this anti-Caveolin-1 antibody can be used to examine the progression of breast and colon cancers and to study functions of Caveolin-1 in human, goat and mouse cells. | - |
dc.language | en | en |
dc.relation.ispartof | Asian-Australasian Journal of Animal Sciences | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Capra hircus; Mus; Oryctolagus cuniculus | - |
dc.title | Development and characterization of a specific anti-Caveolin-1 antibody for Caveolin-1 functional study in human, goat and mouse | - |
dc.type | journal article | en |
dc.identifier.doi | 10.5713/ajas.2007.856 | - |
dc.identifier.scopus | 2-s2.0-34249911216 | - |
item.fulltext | with fulltext | - |
item.grantfulltext | open | - |
dc.relation.pages | 856-865 | - |
dc.relation.journalvolume | 20 | - |
dc.relation.journalissue | 6 | - |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/238490/-1/05.pdf | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | open | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Animal Science and Technology | - |
crisitem.author.dept | Animal Science and Technology | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.orcid | 0000-0002-6557-211X | - |
crisitem.author.orcid | 0000-0002-7701-3163 | - |
crisitem.author.orcid | 0000-0002-5120-6960 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
Appears in Collections: | 動物科學技術學系 |
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