https://scholars.lib.ntu.edu.tw/handle/123456789/96838
Title: | 多氧菌素與葡萄糖之添加對靈芝醱酵液之1,3-β-D-glucan產率及免疫調節功能之影響 Effects of Polyoxin B and glucose on productivity of 1,3-β-glucan of Ganoderma lucidum fermentation broth and Immunopotentiation evaluation |
Authors: | 曾勺瑄 Tseng, Shao-Hsuan |
Keywords: | 靈芝;葡聚醣酶;幾丁質合成酶;Ganoderma;glucanase;chitin synthase | Issue Date: | 2006 | Abstract: | 本研究以液態方式培養靈芝(Ganoderma lucidum),並以靈芝醱酵濾液中具免疫調解活性之1,3-β-D-glucan做為指標成分,探討醱酵條件的改良,最後以靈芝醱酵之多醣產物對U937細胞的間接抑制效果進行免疫調節功效之評估。 實驗中以黑豆5%、黃耆2%、葡萄糖2%作為醱酵基質,於5L醱酵槽進行12天的培養,探討葡萄糖的饋入時間點對指標性成分之影響,由結果可得知第六天饋入1%葡萄糖可在醱酵時間內即時抑制1,3-β-D-glucanase活性,並提高濾液中1,3-β-D-glucan產量1.54倍。在幾丁質合成酶抑制劑Polyoxin B的添加條件之探討上則發現,第八天饋入1.5 ppm可在不嚴重影響菌體生長的情況下,可能藉由促使分泌至胞壁中之結構性多醣無法與幾丁質鍵結而游逸至醱酵濾液中,而達提高濾液中1,3-β-D-glucan產量1.68倍之結果。 於免疫調節功能方面,發現靈芝醱酵多醣產物對於U937細胞之間接抑制能力除了與1,3-β-D-glucan所佔比例有關外,亦受其分子量大小與分支程度之影響,而其中以第六天饋入1%葡萄糖之醱酵條件有最佳之抑制效果,且在其製備之單核細胞條件培養液中亦可偵測到較高之TNF-α、IL-1β與IFN-γ含量。 The objectives of this research were to determine the optimum fermentation condition and to enhance the 1,3-β-D-glucan production of Ganoderma lucidum in submerged culture. The immunopotentiation of the polysaccharide from G. lucidum fermentation was evaluated by the inhibitory ratio of leukemic U937 cell as an model. The basic medium consisted of 5% black soybean, 2% Huangqi and 2% glucose. Furthermore, 1% glucose was added on day 4, 6 and 8, respectively. The effect of feeding time during the 12-day cultivation in 5L fermentor was evaluated. Addition of 1% glucose on day 6 inhibited the 1,3-β-D-glucanase activity and enhanced 1.54-flod 1,3-β-D-glucan in the culture. Moreover, the addition of chitin synthase inhibitor, Polyoxin B (1.5 ppm), probably reduced the binding between the structure glucan and chitin in cell wall, and therefore enhanced 1,3-β-D-glucan content up to 1.68 fold on day 8 without affecting cell growth. The inhibitory ratio of leukemic U937 cell resulted from the G. lucidum polysaccharides was positive correlated to the percentage of 1,3-β-D-glucan, molecular weight and degree of branching. Moreover, significant increases of TNF-α, IL-1β and IFN-γ in the mononuclear condition medium treated with the polysaccharidrs of 1% glucose fed-batch on day 6 were observed. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/56345 | Other Identifiers: | en-US |
Appears in Collections: | 食品科技研究所 |
File | Description | Size | Format | |
---|---|---|---|---|
ntu-95-R93641010-1.pdf | 23.53 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.