https://scholars.lib.ntu.edu.tw/handle/123456789/136522
Title: | Identification and Characterization of a Shrimp White Spot Syndrome Virus (WSSV) Gene That Encodes a Novel Chimeric Polypeptide of Cellular-Type Thymidine Kinase and Thymidylate Kinase | Authors: | Tsai, Meng-Feng Yu, Hon-Tsen Tzeng, Huey-Fen Leu, Jiann-Horng Chou, Chih-Ming Huang, Chang-Jen Wang, Chung-Hsiung Lin, Jung-Yaw Kou, Guang-Hsiung Lo, Chu-Fang |
Keywords: | Penaeus monodon; Taiwan WSSV isolate; Thymidine kinase; Thymidylate kinase; White spot syndrome virus; WSSV tk-tmk gene | Issue Date: | 2000 | Journal Volume: | 277 | Journal Issue: | 1 | Start page/Pages: | 100-110 | Source: | Virology | Abstract: | From previously constructed genomic libraries of a Taiwan WSSV isolate, a putative WSSV tk-tmk gene was identified. Uniquely, the open reading frame (ORF) of this gene was predicted to encode a novel chimeric protein of 388 amino acids with significant homology to two proteins: thymidine kinase (TK) and thymidylate kinase (TMK). Northern blot analysis with a WSSV tk-tmk-specific riboprobe detected a major transcript of 1.6 kb. When healthy adult Penaeus monodon shrimp were inoculated with WSSV, the tk-tmk gene transcript was first detected by RT-PCR analysis at 4 h postinfection and transcription levels continued to increase over the first 18 h. The gene's major in vitro transcription and translation product, equivalent to the predicted size (43 kDa), is a single chimeric protein that includes both the TK and TMK functional motifs. Evidence for phylogenetic analysis and sequence alignment suggested that the gene may have resulted from the fusion of a cellular-type TK gene and a cellular-type TMK gene. Its unique arrangement may also provide a valuable gene marker for WSSV. (C) 2000 Academic Press. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034634328&doi=10.1006%2fviro.2000.0597&partnerID=40&md5=d051aac04182ebd511c19c5171935477 http://ntur.lib.ntu.edu.tw//handle/246246/161585 |
ISSN: | 00426822 | DOI: | 10.1006/viro.2000.0597 | SDG/Keyword: | chimeric protein; polypeptide; thymidine kinase; article; DNA virus; genetic transcription; nonhuman; nucleotide sequence; open reading frame; phylogeny; priority journal; sequence analysis; sequence homology; shrimp; virus characterization; virus gene; White spot syndrome virus; Animalia; Decapoda (Crustacea); DNA viruses; Monodon; Penaeus monodon; Penaeus monodon; Shrimp white spot syndrome virus |
Appears in Collections: | 生命科學系 |
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