https://scholars.lib.ntu.edu.tw/handle/123456789/391221
標題: | Detection of Human α- L -Fucosidases by a Quinone Methide-Generating Probe: Enhanced Activities in Response to Helicobacter pylori Infection | 作者: | N akumar, M. Hsu, Y.-L. Lin, J.C.-Y. Lo, C. Lo, L.-C. Lin, C.-H. LEE-CHIANG LO |
關鍵字: | activity-based probes; fucosidase; Helicobacter pylori; lipopolysaccharides; quinone methide | 公開日期: | 2015 | 卷: | 16 | 期: | 11 | 起(迄)頁: | 1555-1559 | 來源出版物: | ChemBioChem | 摘要: | α-L-Fucosidase activity is associated with several diseases. To study the enzymatic activity change under pathological conditions, we developed a quinone methide-generating activity-based probe useful for examining the presence, activity, and localization of human α-L-fucosidase in vivo in the context of Helicobacter pylori infection. In particular, an increase in intracellular fucosidase (Fuca1) activity was found in gastric epithelial cells upon bacterial infection. We further studied the effect of several bacterial stimulants on this enhanced Fuca1 activity and identified lipopolysaccharides to be a major contributing factor. Probing for answers: We monitored α-L-fucosidase activity in vitro and in vivo using a quinone methide-based probe. The probe was applied for detecting increases in the activity of human fucosidases 1 and 2 in gastric epithelial cells upon H. pylori infection, with lipopolysaccharides identified as a primary contributing factor. ? 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84937250518&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/391221 |
DOI: | 10.1002/cbic.201500178 | SDG/關鍵字: | alpha levo fucosidase; lipopolysaccharide; quinone derivative; alpha levo fucosidase; indole derivative; molecular probe; quinone methide; Article; controlled study; enzyme activity; epithelium cell; Helicobacter infection; human; human cell; in vivo study; priority journal; epithelium; Helicobacter pylori; metabolism; microbiology; molecular probe; physiology; stomach; tumor cell line; Bacteria (microorganisms); Helicobacter pylori; alpha-L-Fucosidase; Cell Line, Tumor; Epithelium; Helicobacter pylori; Humans; Indolequinones; Lipopolysaccharides; Molecular Probes; Stomach |
顯示於: | 化學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。