https://scholars.lib.ntu.edu.tw/handle/123456789/391221
Title: | Detection of Human α- L -Fucosidases by a Quinone Methide-Generating Probe: Enhanced Activities in Response to Helicobacter pylori Infection | Authors: | N akumar, M. Hsu, Y.-L. Lin, J.C.-Y. Lo, C. LEE-CHIANG LO Lin, C.-H. |
Keywords: | activity-based probes; fucosidase; Helicobacter pylori; lipopolysaccharides; quinone methide | Issue Date: | 2015 | Journal Volume: | 16 | Journal Issue: | 11 | Start page/Pages: | 1555-1559 | Source: | ChemBioChem | Abstract: | α-L-Fucosidase activity is associated with several diseases. To study the enzymatic activity change under pathological conditions, we developed a quinone methide-generating activity-based probe useful for examining the presence, activity, and localization of human α-L-fucosidase in vivo in the context of Helicobacter pylori infection. In particular, an increase in intracellular fucosidase (Fuca1) activity was found in gastric epithelial cells upon bacterial infection. We further studied the effect of several bacterial stimulants on this enhanced Fuca1 activity and identified lipopolysaccharides to be a major contributing factor. Probing for answers: We monitored α-L-fucosidase activity in vitro and in vivo using a quinone methide-based probe. The probe was applied for detecting increases in the activity of human fucosidases 1 and 2 in gastric epithelial cells upon H. pylori infection, with lipopolysaccharides identified as a primary contributing factor. ? 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-84937250518&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/391221 |
DOI: | 10.1002/cbic.201500178 | SDG/Keyword: | alpha levo fucosidase; lipopolysaccharide; quinone derivative; alpha levo fucosidase; indole derivative; molecular probe; quinone methide; Article; controlled study; enzyme activity; epithelium cell; Helicobacter infection; human; human cell; in vivo study; priority journal; epithelium; Helicobacter pylori; metabolism; microbiology; molecular probe; physiology; stomach; tumor cell line; Bacteria (microorganisms); Helicobacter pylori; alpha-L-Fucosidase; Cell Line, Tumor; Epithelium; Helicobacter pylori; Humans; Indolequinones; Lipopolysaccharides; Molecular Probes; Stomach |
Appears in Collections: | 化學系 |
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