https://scholars.lib.ntu.edu.tw/handle/123456789/507992
標題: | Evaluation of performance of human immunodeficiency virus antigen/antibody combination assays in Taiwan | 作者: | Chang C.-K. Kao C.-F. Lin P.-H. Huang H.-L. Ho S.-Y. Wong K.-C. Lin B.-C. Yeh C.-C. Lee C.-Y. CHUAN-LIANG KAO Lee C.-N. SUI-YUAN CHANG Yang J.-Y. |
公開日期: | 2017 | 卷: | 50 | 期: | 4 | 起(迄)頁: | 440-447 | 來源出版物: | Journal of Microbiology, Immunology and Infection | 摘要: | Background The fourth-generation human immunodeficiency virus (HIV) combination assay, which can simultaneously detect the presence of anti-HIV antibody and HIV antigen, has been shown to shorten the window period in HIV diagnosis compared with the third-generation HIV antibody immunoassay. This study was aimed to determine the performance of HIV combination assays in Taiwan, where the HIV-1 seroprevalence is 0.007% and HIV-2 infection has never been reported. Methods Performance of three fourth-generation HIV Ag/Ab combination assays (Dia.Pro, Wantai, and Bio-Rad) and one third-generation HIV Ab immunoassay (AxSYM HIV 1/2 gO) was assessed. Results A total of 152 specimens, including 86 confirmed HIV-seropositive and 66 HIV-seronegative samples, were used in the study. The sensitivity of four assays varied from 98.8% to 100%, and specificity varied from 98.5% to 100%. Performance of the 75 equivocal samples, the HIV status of which was confirmed later, in terms of negative prediction varied from 81.8% to 87.5%. The Bio-Rad and Dia.Pro assays exhibited higher sensitivity for the detection of p24 antigen among the three fourth-generation HIV combination assays. Conclusion The three fourth-generation HIV Ag/Ab combination assays exhibited better sensitivity, specificity, and negative prediction than the third-generation HIV Ab immunoassay. ? 2015 |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/507992 | ISSN: | 1684-1182 | DOI: | 10.1016/j.jmii.2015.07.009 | SDG/關鍵字: | antigen p24; Human immunodeficiency virus antibody; Human immunodeficiency virus antigen; Human immunodeficiency virus antibody; Human immunodeficiency virus antigen; antibody detection; antigen detection; Article; controlled study; follow up; HIV test; human; Human immunodeficiency virus 1 group M; Human immunodeficiency virus 1 group O; Human immunodeficiency virus 1 infection; Human immunodeficiency virus 2; Human immunodeficiency virus 2 infection; human immunodeficiency virus antigen antibody combination assay; Human immunodeficiency virus infection; immunoassay; immunoassay analyzer; nonhuman; predictive value; sensitivity and specificity; Taiwan; virus load; Western blotting; blood; comparative study; diagnostic test; evaluation study; Human immunodeficiency virus; Human immunodeficiency virus infection; immunoassay; immunology; isolation and purification; procedures; Diagnostic Tests, Routine; HIV; HIV Antibodies; HIV Antigens; HIV Infections; Humans; Immunoassay; Sensitivity and Specificity; Taiwan |
顯示於: | 醫學檢驗暨生物技術學系 |
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