https://scholars.lib.ntu.edu.tw/handle/123456789/565845
Title: | PspC domain-containing protein (PCP) determines Streptococcus mutans biofilm formation through bacterial extracellular DNA release and platelet adhesion in experimental endocarditis | Authors: | Jung C.-J. Hsu C.-C. JENG-WEI CHEN Cheng H.-W. CHANG-TSU YUAN YU-MIN KUO RON-BIN HSU JEAN-SAN CHIA |
Issue Date: | 1-Feb-2021 | Journal Volume: | 17 | Journal Issue: | 2 | Source: | PLoS Pathogens | Abstract: | Bacterial extracellular DNA (eDNA) and activated platelets have been found to contribute to biofilm formation by Streptococcus mutans on injured heart valves to induce infective endocarditis (IE), yet the bacterial component directly responsible for biofilm formation or platelet adhesion remains unclear. Using in vivo survival assays coupled with microarray analysis, the present study identified a LiaR-regulated PspC domain-containing protein (PCP) in S. mutans that mediates bacterial biofilm formation in vivo. Reverse transcriptase- and chromatin immunoprecipitation-polymerase chain reaction assays confirmed the regulation of pcp by LiaR, while PCP is well-preserved among streptococcal pathogens. Deficiency of pcp reduced in vitro and in vivo biofilm formation and released the eDNA inside bacteria floe along with reduced bacterial platelet adhesion capacity in a fibrinogen-dependent manner. Therefore, LiaR-regulated PCP alone could determine release of bacterial eDNA and binding to platelets, thus contributing to biofilm formation in S. mutans-induced IE. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/565845 | ISSN: | 15537366 | DOI: | 10.1371/JOURNAL.PPAT.1009289 | SDG/Keyword: | chloramphenicol; genomic DNA; spectinomycin; virulence factor; bacterial DNA; bacterial protein; amino acid sequence; Article; bacteremia; bacterial colonization; bacterial endocarditis; bacterial virulence; bacterium identification; biofilm; colony forming unit; endocarditis; immunoprecipitation; microarray analysis; nonhuman; polymerase chain reaction; protein expression; thrombocyte adhesion; animal; extracellular space; genetics; growth, development and aging; host pathogen interaction; human; metabolism; microbiology; normal human; pathology; rat; Streptococcus infection; Streptococcus mutans; thrombocyte adhesion; Animals; Bacterial Proteins; Biofilms; DNA, Bacterial; Endocarditis; Extracellular Space; Healthy Volunteers; Host-Pathogen Interactions; Humans; Platelet Adhesiveness; Rats; Streptococcal Infections; Streptococcus mutans |
Appears in Collections: | 醫學系 |
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