https://scholars.lib.ntu.edu.tw/handle/123456789/641086
標題: | Therapeutic Application of Dendrobium fimbriatum Hook for Retinopathy Caused by Ultraviolet Radiation and Chemotherapy Using ARPE-19 Cells and Mouse Retina | 作者: | Cheng, Chi Feng Wang, Sheue Er Lu, Chen Wen Nguyen, Thi Kim Ngan Shen, Szu Chuan CHIA YING LIEN Chuang, Wu Chang Lee, Ming Chung Wu, Chung Hsin |
關鍵字: | apoptosis | ARPE-19 cells | chemotherapy | Dendrobium fimbriatum | inflammation | mice | oxidative stress | retinopathy | UV | 公開日期: | 1-三月-2024 | 出版社: | MDPI | 卷: | 13 | 期: | 5 | 來源出版物: | Plants | 摘要: | Retinopathy caused by ultraviolet radiation and cancer chemotherapy has increased dramatically in humans due to rapid environmental and social changes. Therefore, it is very important to develop therapeutic strategies to effectively alleviate retinopathy. In China, people often choose dendrobium to improve their eyesight. In this study, we explored how Dendrobium fimbriatum extract (DFE) protects ARPE-19 cells and mouse retinal tissue from damage of ultraviolet (UV) radiation and chemotherapy. We evaluated the antioxidant capacity of DFE using the 1,1-diphenyl-2-trinitophenylhydrazine (DPPH) assay. The protective effects of DEF from UV- and oxaliplatin (OXA)-induced damage were examined in ARPE-19 cells using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and immunofluorescence (IF) stains, and in mouse retinal tissue using immunohistochemistry (IHC) stains. Our results show that DFE has excellent antioxidant capacity. The ARPE-19 cell viability was decreased and the F-actin cytoskeleton structure was damaged by UV radiation and OXA chemotherapy, but both were alleviated after the DFE treatment. Furthermore, DFE treatment can alleviate OXA chemotherapy-induced reduced expressions of rhodopsin and SOD2 and increased expressions of TNF-α and caspase 3 in mouse retinal tissue. Thus, we suggest that DFE can act as suitable treatment for retinopathy through reducing oxidative stress, inflammation, and apoptosis. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/641086 | ISSN: | 2223-7747 | DOI: | 10.3390/plants13050617 |
顯示於: | 體育室 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。