https://scholars.lib.ntu.edu.tw/handle/123456789/325449
標題: | Aqueous nickel-nitrilotriacetate modified Fe3O 4-NH3+ nanoparticles for protein purification and cell targeting | 作者: | Dar-Bin Shieh Chia-Hao Su Fong-Yu Chang Ya-Na Wu Wu-Chou Su Jih Ru Hwu JYH-HORNG CHEN Chen-Sheng Yeh |
公開日期: | 八月-2006 | 卷: | 17 | 期: | 16 | 起(迄)頁: | 4174 - 4182 | 來源出版物: | Nanotechnology | 摘要: | A comprehensive totally aqueous phase synthesis of nickel-nitrilotriacetate (Ni-NTA) modified superparamagnetic Fe3O4 nanoparticles is presented. The Fe3O4-NTA-Ni nanoparticles are able to perform efficient and specific purification of 6-His tagged proteins from crude cell lysates, as evidenced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. The average binding capacity, as demonstrated by streptopain (MW 42kDa), is 0.23mg/mg (protein/Fe3O4-NTA-Ni). Considering the high affinity and specificity of the binding between hexahistidine motif and Ni-NTA, Ni-NTA modified nanoparticles could act as a module to carry 6-His tagged proteins on the particle surface with molecular orientation control, since only the 6-His domain could be attached. These modularly designed functional nanoparticles enhance cancer cell targeting, as supported by the in vitro receptor mediated targeting assay using RGD-4C-6-His fusion peptide. The nanoparticles show no significant hemolysis for human blood and could be investigated further for their in vivo functional imaging applications. ? 2006 IOP Publishing Ltd. |
URI: | http://scholars.lib.ntu.edu.tw/handle/123456789/325449 https://www.scopus.com/inward/record.uri?eid=2-s2.0-33748898326&doi=10.1088%2f0957-4484%2f17%2f16%2f030&partnerID=40&md5=f42b089f077c55eb332f680104c40bf4 |
ISSN: | 09574484 | DOI: | 10.1088/0957-4484/17/16/030 | SDG/關鍵字: | Blood; Iron compounds; Molecular orientation; Nickel; Polypeptides; Proteins; Hemolysis; Nanoparticles; Nickel-nitrilotriacetate (Ni-NTA); Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE); Nanostructured materials |
顯示於: | 電機工程學系 |
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