https://scholars.lib.ntu.edu.tw/handle/123456789/405931
Title: | Lectin-functionalized mesoporous silica nanoparticles for endoscopic detection of premalignant colonic lesions | Authors: | Chen N.-T. Souris J.S. Cheng S.-H. Chu C.-H. Wang Y.-C. Konda V. Dougherty U. Bissonnette M. Mou C.-Y. Chen C.-T. Lo L.-W. |
Keywords: | Colorectal tumors; Endoscopic; Mesoporous silica nanoparticle; Ulex europaeus Agglutinin 1 (UEA1) | Issue Date: | 2017 | Journal Volume: | 13 | Journal Issue: | 6 | Start page/Pages: | 1941-1952 | Source: | Nanomedicine: Nanotechnology, Biology, and Medicine | Abstract: | Colorectal cancer (CRC) is one of the leading causes of cancer-deaths worldwide. Methods for the early in situ detection of colorectal adenomatous polyps and their precursors – prior to their malignancy transformation into CRC – are urgently needed. Unfortunately at present, the primary diagnostic method, colonoscopy, can only detect polyps and carcinomas by shape/morphology; with sessile polyps more likely to go unnoticed than polypoid lesions. Here we describe our development of polyp-targeting, fluorescently-labeled mesoporous silica nanoparticles (MSNs) that serve as targeted endoscopic contrast agents for the early detection of colorectal polyps and cancer. In vitro cell studies, ex vivo histopathological analysis, and in vivo colonoscopy and endoscopy of murine colorectal cancer models, demonstrate significant binding specificity of our nanoconstructs to pathological lesions via targeting aberrant α-L-fucose expression. Our findings strongly suggest that lectin-functionalized fluorescent MSNs could serve as a promising endoscopic contrast agent for in situ diagnostic imaging of premalignant colonic lesions. ? 2017 Elsevier Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020873707&doi=10.1016%2fj.nano.2017.03.014&partnerID=40&md5=c343f942c47044ca490781e2d50ebe29 https://scholars.lib.ntu.edu.tw/handle/123456789/405931 |
DOI: | 10.1016/j.nano.2017.03.014 | SDG/Keyword: | Diseases; Endoscopy; Proteins; Silica nanoparticles; Binding specificities; Colorectal cancers (CRC); Colorectal tumors; Endoscopic; Histopathological analysis; Mesoporous silica nanoparticles; Pathological lesions; Ulex europaeus Agglutinin 1 (UEA1); Mesoporous materials; fluorescent dye; fucose; lectin; mesoporous silica nanoparticle; lectin; nanoparticle; silicon dioxide; animal experiment; animal model; animal tissue; Article; Caco-2 cell line; colonoscopy; colorectal cancer; colorectal polyp; controlled study; early cancer diagnosis; endoscopy; ex vivo study; HCT 116 cell line; histopathology; human; human cell; human tissue; in vivo study; male; mouse; nonhuman; precancer; A J mouse; animal; chemically induced; chemistry; colon; colon polyp; colorectal tumor; pathology; precancer; procedures; tumor cell culture; Animals; Colon; Colonic Polyps; Colonoscopy; Colorectal Neoplasms; Endoscopy; Fluorescent Dyes; Humans; Lectins; Male; Mice; Mice, Inbred A; Nanoparticles; Precancerous Conditions; Silicon Dioxide; Tumor Cells, Cultured |
Appears in Collections: | 化學系 |
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Lectin-functionalized mesoporous silica nanoparticles for endoscopic detection of premalignant colonic lesions.pdf | 2.13 MB | Adobe PDF | View/Open |
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