https://scholars.lib.ntu.edu.tw/handle/123456789/506075
標題: | A microfluidic microwell device for immunomagnetic single-cell trapping | 作者: | Huang, N.-T. Hwong, Y.-J. Lai, R.L. NIEN-TSU HUANG |
關鍵字: | Immunomagnetic; Microfluidics; Microwell; Single-cell trapping | 公開日期: | 2018 | 卷: | 22 | 期: | 2 | 來源出版物: | Microfluidics and Nanofluidics | 摘要: | Single-cell analysis has been widely applied in various biomedical applications, such as cancer diagnostics, immune status monitoring, and drug screening. To perform an accurate and rapid cellular analysis, various magnetic-activated cell sorting techniques are available in the markets. However, large sample requirement and uneven magnetic field distribution limit its application in single-cell trapping and following analysis. To address these problems, we developed a microfluidic microwell device for immunomagnetic single-cell trapping. By adding a microwell layer between the microchannel and magnet, the magnetic field along the device becomes more uniform. Besides, magnetic beads can be retained in the array of microwell after the high-speed washing step, whereas untrapped beads would be flushed away, resulting in high single-particle trapping efficiency (62%) and purity (99.6%). To achieve large-area single-cell trapping, we introduced a “sweeping” loading protocol to further expand the single-particle trapping range. In the microwell region near to the edge of the magnet, over 3000 single magnetic beads were trapped in a 10?mm2 area. Finally, we demonstrated immunomagnetic-labeled THP-1 cells can successfully be trapped at single-cell level in the microwell. The cell trapping process can be done in 10?min. We believe the platform with an accurate and efficient single-cell trapping functionality could potentially be used for various cellular analyses at the single-cell level. ? 2018, Springer-Verlag GmbH Germany, part of Springer Nature. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/506075 | DOI: | 10.1007/s10404-018-2040-x | SDG/關鍵字: | Cytology; Magnetic fields; Magnetism; Magnets; Medical applications; Microchannels; Microfluidics; Biomedical applications; Cancer diagnostics; Immunomagnetic; Loading protocols; Magnetic field distribution; Micro wells; Single-cell trapping; Singlecell analysis; Cells |
顯示於: | 電機工程學系 |
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