https://scholars.lib.ntu.edu.tw/handle/123456789/517380
標題: | Integrated optical coherence tomography and optical coherence microscopy imaging of ex vivo human renal tissues | 作者: | HSIANG-CHIEH LEE Zhou C. Cohen D.W. Mondelblatt A.E. Wang Y. Aguirre A.D. Shen D. Sheikine Y. Fujimoto J.G. Connolly J.L. Zhou C. Cohen D.W. Mondelblatt A.E. Wang Y. Aguirre A.D. Shen D. Sheikine Y. Fujimoto J.G. Connolly J.L. |
關鍵字: | imaging; kidney; kidney neoplasms; microscopy; optical coherence; three-dimensional; tomography | 公開日期: | 2012 | 卷: | 187 | 期: | 2 | 起(迄)頁: | 691-699 | 來源出版物: | Journal of Urology | 摘要: | We evaluated the feasibility of using optical coherence tomography and optical coherence microscopy technology to assess human kidney morphology. A total of 35 renal specimens from 19 patients, consisting of 12 normal tissues and 23 tumors (16 clear cell renal cell carcinomas, 5 papillary renal cell carcinomas and 2 oncocytomas) were imaged ex vivo after surgical resection. Optical coherence tomography and optical coherence microscopy images were compared to corresponding hematoxylin and eosin histology to identify characteristic features of normal and pathological renal tissues. Three pathologists blinded to histology evaluated the sensitivity and specificity of optical coherence microscopy images to differentiate normal from neoplastic renal tissues. Optical coherence tomography and optical coherence microscopy images of normal kidney revealed architectural features, including glomeruli, convoluted tubules, collecting tubules and loops of Henle. Each method of imaging renal tumors clearly demonstrated morphological changes and decreased imaging depth. Optical coherence tomography and microscopy features matched well with the corresponding histology. Three observers achieved 88%, 100% and 100% sensitivity, and 100%, 88% and 100% specificity, respectively, when evaluating normal vs neoplastic specimens using optical coherence microscopy images with substantial interobserver agreement (κ = 0.82, p <0.01). Integrated optical coherence tomography and optical coherence microscopy imaging provides coregistered, multiscale images of renal pathology in real time without exogenous contrast medium or histological processing. High sensitivity and specificity were achieved using optical coherence microscopy to differentiate normal from neoplastic renal tissues, suggesting possible applications for guiding renal mass biopsy or evaluating surgical margins. © 2012 American Urological Association Education and Research, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84855571893&doi=10.1016%2fj.juro.2011.09.149&partnerID=40&md5=4cf484f60c3111796e4322df79b2ff51 https://scholars.lib.ntu.edu.tw/handle/123456789/517380 |
DOI: | 10.1016/j.juro.2011.09.149 | SDG/關鍵字: | eosin; hematoxylin; adult; aged; article; cancer tissue; clear cell renal cell carcinoma; clinical article; clinical assessment; controlled study; diagnostic test accuracy study; ex vivo study; feasibility study; female; glomerulus; Henle loop; histopathology; human; human tissue; image quality; kidney; kidney carcinoma; kidney collecting tubule; kidney distal tubule; kidney papilla; kidney parenchyma; kidney surgery; male; microscopy; oncocytoma; optical coherence microscopy; optical coherence tomography; papillary renal cell carcinoma; pathogenesis; pathologist; priority journal; sensitivity and specificity; surgical technique; tissue structure; Adenoma, Oxyphilic; Adult; Aged; Carcinoma, Renal Cell; Humans; Kidney Neoplasms; Middle Aged; Tomography, Optical Coherence |
顯示於: | 電機工程學系 |
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