https://scholars.lib.ntu.edu.tw/handle/123456789/523547
Title: | AS1411 aptamer-conjugated Gd2O3:Eu nanoparticles for target-specific computed tomography/magnetic resonance/fluorescence molecular imaging | Authors: | Kuo T. Lai W. Li C. Wun Y. Chang H. Chen J. PAN-CHYR YANG Chen C. |
Keywords: | aptamer; contrast agent; molecular imaging; nanoparticles synthesis | Issue Date: | 2014 | Publisher: | Tsinghua University Press | Journal Volume: | 7 | Journal Issue: | 5 | Start page/Pages: | 658-669 | Source: | Nano Research | Abstract: | Europium-doped gadolinium oxide (Gd2O3:Eu) nanoparticles have been synthesized, and then their surfaces have been conjugated with nucleolintargeted AS1411 aptamer to form functionalized target-specific Gd2O3:Eu nanoparticles (A-GdO:Eu nanoparticles). The A-GdO:Eu nanoparticles present strong fluorescence in the visible range, high magnetic susceptibility, X-ray attenuation and good biocompatibility. The A-GdO:Eu nanoparticles have been applied to test molecular expression of nucleolin highly expressed CL1-5 lung cancer cells under a confocal microscope. Fluorescence imaging clearly reveals that the nanoparticles can be applied as fluorescent tags for cancer-targeting molecular imaging. Furthermore, taking together their excellent T 1 contrast and strong computed tomography (CT) signal, the A-GdO:Eu nanoparticles demonstrate a great capability for use as a dual modality contrast agent for CT and magnetic resonance (MR) molecular imaging. Animal experiments also show that the A-GdO:Eu nanoparticles are able to contrast the tissues of BALB/c mice using CT modality. Moreover, the obvious red fluorescence of A-GdO:Eu nanoparticles can be visualized in a tumor by the naked eye. Overall, our results demonstrate that the A-GdO:Eu nanoparticles can not only serve as new medical contrast agents but also as intraoperative fluorescence imaging probes for guided surgery in the near future. [Figure not available: see fulltext.] ? 2014 Tsinghua University Press and Springer-Verlag Berlin Heidelberg. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84900561094&doi=10.1007%2fs12274-014-0420-4&partnerID=40&md5=84d6adb034e4fde09f5ab2baefa344a1 https://scholars.lib.ntu.edu.tw/handle/123456789/523547 |
ISSN: | 1998-0124 | DOI: | 10.1007/s12274-014-0420-4 |
Appears in Collections: | 醫學系 |
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