https://scholars.lib.ntu.edu.tw/handle/123456789/577048
Title: | Library preparation for next-generation sequencing using a multiplex microfluidic chip | Authors: | Hsu P.-W Chen C.-L Tseng H.-W Wo A.M. ANDREW WO |
Keywords: | Centrifugation; Fluidic devices; Automated handling; Centrifugal microfluidics; Genomic analysis; Low sample input; Microfluidic chip; Next-generation sequencing; Sample preparation; Substantial reduction; Microfluidics | Issue Date: | 2018 | Journal Volume: | 4 | Start page/Pages: | 2355-2357 | Source: | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 | Abstract: | The emergence of next-generation sequencing (NGS) has propelled genomic analysis into a new era. However, the bottleneck is the library preparation which, for most cases, is labor-intensive and operator-dependent. In this study, a centrifugal-based microfluidic design enables on-chip fragmentation, end repair, A-addition, and ligation. Key features include multiplex processing, substantial reduction in reagent usage, low (?250 pg) input DNA quantity and reliable fluidic control. With simple to operate and low-cost microfluidic chip, the design demonstrated the potential for automated handling for multiplex library preparation for various applications. Copyright ? (2018) by Chemical and Biological Microsystems Society. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079780161&partnerID=40&md5=29ea3517bfb82bd8bde8338baa02ac0d https://scholars.lib.ntu.edu.tw/handle/123456789/577048 |
Appears in Collections: | 應用力學研究所 |
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