https://scholars.lib.ntu.edu.tw/handle/123456789/592676
標題: | Upregulation of interleukin-1 by Epstein-Barr virus latent membrane protein 1 and its possible role in nasopharyngeal carcinoma cell growth | 作者: | Huang Y.-T. Liu M.-Y. CHING-HWA TSAI TE-HUEI YEH |
公開日期: | 2010 | 卷: | 32 | 期: | 7 | 起(迄)頁: | 869-876 | 來源出版物: | Head and Neck | 摘要: | Background. Nasopharyngeal carcinoma (NPC) is associated with Epstein-Barr virus (EBV) infection. We previously found that interleukin (IL)-1α and IL-1β significantly increased in NPC tissues. This study investigated what EBVencoded proteins were involved in such IL-1 production. Methods and Results. IL-1α and IL-1β messenger ribonucleic acids (mRNAs) were detected in the EBV latent membrane protein 1 (LMP1) transfectant (LMP135) only by reverse transcriptase-polymerase chain reaction (RT-PCR). LMP1-mediated IL-1α and IL-1β production could be enhanced by tumor necrosis factor alpha (TNF-α), determined by enzymelinked immunosorbent assay (ELISA). Moreover, IL-1α and IL-1β mRNAs and proteins were increased in a dose-dependent manner in epithelial cells transiently transfected by an LMP1 plasmid. Besides, immortalized human epidermal keratinocyte (RHEK-1) epithelial cells could be enhanced to proliferate by IL-1α and IL-1β determined by water-soluble tetrazolium salt (WST-1) assay. Conclusions. EBV LMP1 is capable of upregulating IL-1α and IL-1β secretions from epithelial cells and positively modulated by TNF-a. This may consequently contribute to tumor growth in patients with NPC. ? 2009 Wiley Periodicals, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-77954412016&doi=10.1002%2fhed.21270&partnerID=40&md5=943b5b118c353221e6622cc85dfe9d6d https://scholars.lib.ntu.edu.tw/handle/123456789/592676 |
ISSN: | 1043-3074 | DOI: | 10.1002/hed.21270 | SDG/關鍵字: | interleukin 1alpha; interleukin 1beta; latent membrane protein 1; messenger RNA; tumor necrosis factor alpha; article; cancer cell; cell growth; cell proliferation; controlled study; cytokine production; dose response; enzyme linked immunosorbent assay; epithelium cell; Epstein Barr virus; human; human cell; keratinocyte; nasopharynx carcinoma; nonhuman; priority journal; real time polymerase chain reaction; reverse transcription polymerase chain reaction; upregulation; Carcinoma; Cell Culture Techniques; Cell Line; Cell Proliferation; Epithelial Cells; Herpesvirus 4, Human; Humans; Interleukin-1; Nasopharyngeal Neoplasms; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Viral Matrix Proteins |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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