https://scholars.lib.ntu.edu.tw/handle/123456789/606878
標題: | Thermally driven formation of polyphenolic carbonized nanogels with high anticoagulant activity from polysaccharides | 作者: | Mao J.-Y Unnikrishnan B Chu H.-W Harroun S.G Chen Y.-R Wu A.-T Chang H.-T Lin H.-J Huang C.-C. HUAN-TSUNG CHANG |
關鍵字: | Binding energy;Biocompatibility;Blood;Carbonization;Dissociation;Enzymes;Flavonoids;Nanostructured materials;Polyphenolic compounds;Anticoagulant activities;Binding affinities;Carbonization process;Dissociation constant;Heating process;Sulfated polysaccharides;Thermally driven;Thrombotic disorders;Alginate;alginic acid;anticoagulant agent;blood clotting factor 11;blood clotting factor 12;blood clotting factor 5;blood clotting factor 7;caffeic acid;catechin;enoxaparin;ferulic acid;fucoidin;graphene like nanosheet polyphenolic alginate nanogel;kaolin;nanogel;naringenin;quercetin;thrombin;unclassified drug;polysaccharide;activated partial thromboplastin time;animal experiment;animal model;anticoagulation;Article;binding affinity;biocompatibility;bleeding;blood clotting;carbonization;controlled study;cytotoxicity;embryo;ex vivo study;human;human cell;in vivo study;male;nonhuman;prothrombin time;rat;sulfation;tail bleeding time;thrombin time;thromboelastography;animal;Animals;Anticoagulants;Blood Coagulation;Nanogels;Polysaccharides;Rats;Thrombin | 公開日期: | 2021 | 卷: | 9 | 期: | 13 | 起(迄)頁: | 4679-4690 | 來源出版物: | Biomaterials Science | 摘要: | We have demonstrated that alginate with negligible anticoagulant activity can be converted into carbonized nanogels with potent anticoagulant activity through a solid-state heating process. The conversion of alginate into graphene-like nanosheet (GNS)-embedded polyphenolic-alginate nanogels (GNS/Alg-NGs) has been carried out through condensation and carbonization processes. The GNS/Alg-NGs exhibit much stronger anticoagulant activity (>520-fold) compared to untreated alginate, mainly because their polyphenolic structures have a high binding affinity [dissociation constant (Kd) = 2.1 × 10-10 M] toward thrombin. In addition, the thrombin clotting time delay caused by the GNS/Alg-NGs is 10-fold longer than that of natural polyphenolic compounds, such as quercetin, catechin, naringenin, caffeic acid, and ferulic acid. The thrombin-or kaolin-activated thromboelastography of whole-blood coagulation reveals that the GNS/Alg-NGs display a much stronger anticoagulant ability than that of untreated alginate and naturally sulfated polysaccharides (fucoidan). The GNS/Alg-NGs exhibit superior biocompatibility and anticoagulant activity, as observed with an in vivo rat model, revealing their potential as a blood thinner for the treatment of thrombotic disorders. ? The Royal Society of Chemistry. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85109105253&doi=10.1039%2fd1bm00402f&partnerID=40&md5=e44907494f86badf0630f9a2491840c7 https://scholars.lib.ntu.edu.tw/handle/123456789/606878 |
ISSN: | 20474830 | DOI: | 10.1039/d1bm00402f |
顯示於: | 化學系 |
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